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Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts

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Main Authors: Noorhaslina, Hashim, Abdul Razak, Shaari, Awang Soh, Mamat, Syarhabil, Ahmad
Other Authors: leein16@gmail.com
Format: Article
Language:English
Published: EDP Sciences 2020
Subjects:
Orthosiphon stamineus
Bioactive compounds
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69149
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spelling my.unimap-691492020-12-23T09:22:59Z Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts Noorhaslina, Hashim Abdul Razak, Shaari Awang Soh, Mamat Syarhabil, Ahmad leein16@gmail.com Orthosiphon stamineus Bioactive compounds Link to publisher's homepage at https://www.matec-conferences.org/ Bioactive compounds of Orthosiphon stamineus have been known to have the beneficial effects on health. Efficient extraction of these beneficial compounds is very important. The study was conducted to determine the efficiency of methanol as an extraction solvent for bioactive compound extraction of O. stamineus raw materials. Leaf sample was extracted in 25, 50 and 100% methanol and distilled water for 2, 4 and 8 hours at 40°C. The free radical scavenging activity (FRSA) method was used to determine antioxidant capacity and Follin-Ciocalteu method was used to determine samples total phenolic compounds. The quantification of bioactive constituents by using the high performance liquid chromatography (HPLC) and the standard markers was used for this analysis such as rosmarinic acid (RA), sinensetin (SEN) and 3-hydroxy-5,6,7,4-tetramethoxyflavone (TMF). The current research shows that the yield of RA concentration was high in 50% methanol extracts at 2, 4 and 8 h of extraction. For the FRSA result showed that methanol at 50% and 100% was the most effective concentration levels inhibition between 45-55% at 8 hours’ time extraction. For the total phenolic compound, the 50% methanol concentration in O. stamineus leaf had the highest value compared with 0%, 25% and 100% methanol concentration. But, it was observed that time extraction did not influence the TPC values for different concentrations of methanolic extracts. These indicated that time extraction had affected the total capacities of antioxidant and 50% methanolic extracts was the best concentration for indicated the TPC in the O. Storminess leaf. 2020-12-23T09:22:59Z 2020-12-23T09:22:59Z 2016 Article MATEC Web Conferences, vol.78, 2016, 7 pages 2261-236X (online) http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69149 https://doi.org/10.1051/matecconf/20167801004 en 2nd International Conference on Green Design and Manufacture 2016 (IConGDM 2016); EDP Sciences
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Orthosiphon stamineus
Bioactive compounds
spellingShingle Orthosiphon stamineus
Bioactive compounds
Noorhaslina, Hashim
Abdul Razak, Shaari
Awang Soh, Mamat
Syarhabil, Ahmad
Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
description Link to publisher's homepage at https://www.matec-conferences.org/
author2 leein16@gmail.com
author_facet leein16@gmail.com
Noorhaslina, Hashim
Abdul Razak, Shaari
Awang Soh, Mamat
Syarhabil, Ahmad
format Article
author Noorhaslina, Hashim
Abdul Razak, Shaari
Awang Soh, Mamat
Syarhabil, Ahmad
author_sort Noorhaslina, Hashim
title Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
title_short Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
title_full Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
title_fullStr Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
title_full_unstemmed Effect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extracts
title_sort effect of differences methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of orthosiphon stamineus extracts
publisher EDP Sciences
publishDate 2020
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69149
_version_ 1698698566418038784
score 13.214268

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