Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues
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Universiti Malaysia Perlis (UniMAP)
2020
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my.unimap-677132020-09-28T03:08:02Z Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues Chin, Fhong Soon Phriya, Mohana Elfa Rizan, Rizon Intan Sue Liana, Abdul Hamid Naznin, Sultana Marlia, Morsin Kian, Sek Tee Gim, Pao Lim Mohd Khairul, Ahmad Nafarizal, Nayan soon@uthm.edu.my Atmospheric pressure plasma jet Microspheroids Microtissues Non-thermal Cell viability Link to publisher's homepage at http://ijneam.unimap.edu.my Non-thermal atmospheric plasma jet (NTAPPJ) is believed to be effective in treating monolayer cells cultured in-vitro due to the oxidative stresses induced. However, it is controversial whether studying the impact of plasma to cells in monolayer can be representative to a model close to the in-vivo tissues. This study revealed and compared the effects of NTAPPJ treatment directly to both monolayer of HeLa cells and microspheroids in the culture media, at different exposure time from 15 to 60 seconds at a fixed power of 80 W. Optical emission spectroscopy (OES) revealed that the plasma applied to the cells is rich in hydroxyl, nitrogen, oxygen and argon species. The experimental results revealed that the treatment time from 15 seconds onwards was detrimental to the monolayer of cells, but not to the spheroids compared with the negative controls. After long NTAPPJ exposure, the HeLa cells in the spheroids were found viable, regardless of the plasma exposure time applied. The multicellular structure of the spheroids has prevented the penetration of free radicals into the cells encapsulated in the spheroids. 2020-09-28T03:08:02Z 2020-09-28T03:08:02Z 2020-07 Article International Journal of Nanoelectronics and Materials, vol.13(3), 2020, pages 473-482 1985-5761 (Printed) 1997-4434 (Online) http://dspace.unimap.edu.my:80/xmlui/handle/123456789/67713 http://ijneam.unimap.edu.my en Universiti Malaysia Perlis (UniMAP) |
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Atmospheric pressure plasma jet Microspheroids Microtissues Non-thermal Cell viability |
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Atmospheric pressure plasma jet Microspheroids Microtissues Non-thermal Cell viability Chin, Fhong Soon Phriya, Mohana Elfa Rizan, Rizon Intan Sue Liana, Abdul Hamid Naznin, Sultana Marlia, Morsin Kian, Sek Tee Gim, Pao Lim Mohd Khairul, Ahmad Nafarizal, Nayan Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
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Link to publisher's homepage at http://ijneam.unimap.edu.my |
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soon@uthm.edu.my |
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soon@uthm.edu.my Chin, Fhong Soon Phriya, Mohana Elfa Rizan, Rizon Intan Sue Liana, Abdul Hamid Naznin, Sultana Marlia, Morsin Kian, Sek Tee Gim, Pao Lim Mohd Khairul, Ahmad Nafarizal, Nayan |
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Article |
| author |
Chin, Fhong Soon Phriya, Mohana Elfa Rizan, Rizon Intan Sue Liana, Abdul Hamid Naznin, Sultana Marlia, Morsin Kian, Sek Tee Gim, Pao Lim Mohd Khairul, Ahmad Nafarizal, Nayan |
| author_sort |
Chin, Fhong Soon |
| title |
Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
| title_short |
Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
| title_full |
Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
| title_fullStr |
Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
| title_full_unstemmed |
Effects of atmospheric pressure plasma jet treatment duration to the viability of Hela cells and microtissues |
| title_sort |
effects of atmospheric pressure plasma jet treatment duration to the viability of hela cells and microtissues |
| publisher |
Universiti Malaysia Perlis (UniMAP) |
| publishDate |
2020 |
| url |
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/67713 |
| _version_ |
1698698356131364864 |
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13.159267 |