Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
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2009
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my.unimap-66732009-08-04T07:43:53Z Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) A.L., Ahmad C.J.C., Derek Mohamed Zulkali, Mohamed Daud Thaumatin Aqueous two-phase system Polyethylene glycol/sodium sulfate system Two-level fractional factorial design Response surface methodology Polyethylene glycol Polyethylene glycol -- Biotechnology Separation (Technology) Link to publisher's homepage at www.elsevier.com The work attempts to study and optimize aqueous two-phase system (ATPS) made of polyethylene glycol (PEG)/sodium sulfate and PEG/potassium phosphate. Five factors (PEG's molecular weight, PEG's concentration, pH, concentration of sodium chloride and phase-forming salt) affecting the thaumatin partitioning were studied. A two-level fractional factorial was initially carried out. The statistical analysis showed that for both the systems, the phase-forming salt concentration and the concentration of sodium chloride significantly affects the K value for thaumatin partitioning. However, the performance of PEG/sodium sulfate system was generally better than the PEG/potassium phosphate system. Hence a detail study was carried out on the PEG/sodium sulfate system using central composite rotatable design (CCRD) in response surface methodology (RSM). It was observed that high salt concentration increased the K value by sevenfold and the PEG molecular weight was not a significant factor. The optimal condition gave a K value for thaumatin partitioning of 24.15 with a yield of 96.02%. 2009-08-04T07:43:29Z 2009-08-04T07:43:29Z 2008-09-22 Article Separation and Purification Technology, vol.62 (3), 2008, pages 702-708. 13835866 http://www.sciencedirect.com/science/journal/13835866 http://hdl.handle.net/123456789/6673 en Elsevier B.V. |
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Thaumatin Aqueous two-phase system Polyethylene glycol/sodium sulfate system Two-level fractional factorial design Response surface methodology Polyethylene glycol Polyethylene glycol -- Biotechnology Separation (Technology) |
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Thaumatin Aqueous two-phase system Polyethylene glycol/sodium sulfate system Two-level fractional factorial design Response surface methodology Polyethylene glycol Polyethylene glycol -- Biotechnology Separation (Technology) A.L., Ahmad C.J.C., Derek Mohamed Zulkali, Mohamed Daud Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| description |
Link to publisher's homepage at www.elsevier.com |
| format |
Article |
| author |
A.L., Ahmad C.J.C., Derek Mohamed Zulkali, Mohamed Daud |
| author_facet |
A.L., Ahmad C.J.C., Derek Mohamed Zulkali, Mohamed Daud |
| author_sort |
A.L., Ahmad |
| title |
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| title_short |
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| title_full |
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| title_fullStr |
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| title_full_unstemmed |
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) |
| title_sort |
optimization of thaumatin extraction by aqueous two-phase system (atps) using response surface methodology (rsm) |
| publisher |
Elsevier B.V. |
| publishDate |
2009 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/6673 |
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1643788537128026112 |
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13.214268 |