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Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model

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Bibliographic Details
Main Author: Nooramalina, Azhar
Other Authors: Dr. Khairul Farihan Kasim
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2019
Subjects:
Silver nanoparticles
Piper betle
Antimicrobial
Antimicrobial properties
Phyto-pathogen model
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/59928
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spelling my.unimap-599282023-04-04T02:13:17Z Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model Nooramalina, Azhar Dr. Khairul Farihan Kasim Silver nanoparticles Piper betle Antimicrobial Antimicrobial properties Phyto-pathogen model Access is limited to UniMAP community. Silver nanoparticles are well known as one of the promising agent especially for antibacterial activity. This project was aimed to evaluate the synthesized silver nanoparticles from Piper betle and their antimicrobial properties against phyto-pathogen model. The formation of silver nanoparticles in aqueous extract were confirmed by colour changes from yellowish to brown. To further confirm the presence of silver nanoparticles, UV-Vis spectrum was used and the absorbance of the silver nanoparticles were analysed at 452 nm. Antimicrobial activity of the synthesized silver nanoparticles of P. bettle was evaluated by using agar well plate on Escherichia coli (gram-negative bacteria), Pseudomonas aeruginasa (gram-positive bacteria) and the Aspergillus niger (fungi). The results show that synthesized silver nanoparticles on 1 mM concentration exhibits admirable zone of inhibition against E. coli, P. aeruginasa and A. niger (14.1±0.13, 14.5±0.17 and 14.7±0.40 mm, respectively). All the synthesized silver nanoparticles show significant antimicrobial activity compared to the aqueous extract of P. bettle. Minimum inhibition concentration (MIC) of the best synthesized silver nanoparticles of P. bettle (1 Mm) was further evaluated and shows that P. aeruginasa, E. coli and A. niger was perceived at three-fold, two-fold and one-fold dilution from the original samples respectively. In order to characterize the size and shape of silver nanoparticles, field emission scanning electron microscope (FESEM) was used and revealed that silver nanoparticles were in spherical shape that range from 15 to 19 nm in size. The presence of elemental silver was obtained by using energy-dispersive spectroscopy (EDX), which 2019-05-10T07:54:05Z 2019-05-10T07:54:05Z 2017-06 Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/59928 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Silver nanoparticles
Piper betle
Antimicrobial
Antimicrobial properties
Phyto-pathogen model
spellingShingle Silver nanoparticles
Piper betle
Antimicrobial
Antimicrobial properties
Phyto-pathogen model
Nooramalina, Azhar
Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
description Access is limited to UniMAP community.
author2 Dr. Khairul Farihan Kasim
author_facet Dr. Khairul Farihan Kasim
Nooramalina, Azhar
format Thesis
author Nooramalina, Azhar
author_sort Nooramalina, Azhar
title Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_short Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_full Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_fullStr Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_full_unstemmed Green synthesis of silver nanoparticles using Piper Betle leaf extracts and their antimicrobial properties against isolated Phyto-pathogen model
title_sort green synthesis of silver nanoparticles using piper betle leaf extracts and their antimicrobial properties against isolated phyto-pathogen model
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2019
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/59928
_version_ 1772813059522297856
score 13.214268

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