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Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia

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Main Authors: Mansur, Rusnam, Gusmanizar, Neni, Farrah Aini, Dahalan, Noor Azlina, Masdor, Siti Aqlima, Ahmad, Mohd Shukri, Shukor, Muhamad Akhmal Hakim, Roslan, Mohd Yunus, Shukor
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: Institute of Integrative Omics and Applied Biotechnology (IIOAB) 2016
Subjects:
Acetamide
Acrylamide
Burkholderia sp
Molybdenum blue
Molybdenum reduction
Propionamide
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41252
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spelling my.unimap-412522016-04-04T03:07:56Z Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia Mansur, Rusnam Gusmanizar, Neni Farrah Aini, Dahalan Noor Azlina, Masdor Siti Aqlima, Ahmad Mohd Shukri, Shukor Muhamad Akhmal Hakim, Roslan Mohd Yunus, Shukor farrahaini@unimap.edu.my rusnam_ms@yahoo.com Acetamide Acrylamide Burkholderia sp Molybdenum blue Molybdenum reduction Propionamide Link to publisher's homepage at http://www.iioab.org/ A molybdenum-reducing bacterium isolated from contaminated soil was able to utilize acrylamide as the electron donor source, and was able utilize acrylamide, acetamide and propionamide for growth. Reduction was optimal at pH between 6.0 to 6.3, at temperatures of between 30 and 37 oC, glucose as the electron donor, phosphate at 5.0 mM, and sodium molybdate at 15 mM. The absorption spectrum of the Mo-blue indicates it is a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury (ii), silver (i) and chromium (vi) at 2 p.p.m. by 91.9, 82.7 and 17.4 %, respectively. Biochemical analysis resulted in a tentative identification of the bacterium as Burkholderia cepacia strain Neni-11. The growth of this bacterium modelled according to the modified Gompertz model. The growth parameters obtained were maximum specific growth rates of 1.241 d-1, 0.971 d-1, 0.85 d-1 for acrylamide, propionamide and acetamide, respectively, while the lag periods of 1.372 d, 1.562 and 1.639 d were observed for acrylamide, propionamide and acetamide, respectively. The ability of this bacterium to detoxify molybdenum and grown on toxic amides makes this bacterium an important tool for bioremediation. 2016-04-04T03:07:56Z 2016-04-04T03:07:56Z 2016-01-01 Article IIOAB Journal, vol. 7 (1), 2016, pages 28-40 0976-3104 http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41252 en Institute of Integrative Omics and Applied Biotechnology (IIOAB)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Acetamide
Acrylamide
Burkholderia sp
Molybdenum blue
Molybdenum reduction
Propionamide
spellingShingle Acetamide
Acrylamide
Burkholderia sp
Molybdenum blue
Molybdenum reduction
Propionamide
Mansur, Rusnam
Gusmanizar, Neni
Farrah Aini, Dahalan
Noor Azlina, Masdor
Siti Aqlima, Ahmad
Mohd Shukri, Shukor
Muhamad Akhmal Hakim, Roslan
Mohd Yunus, Shukor
Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
description Link to publisher's homepage at http://www.iioab.org/
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Mansur, Rusnam
Gusmanizar, Neni
Farrah Aini, Dahalan
Noor Azlina, Masdor
Siti Aqlima, Ahmad
Mohd Shukri, Shukor
Muhamad Akhmal Hakim, Roslan
Mohd Yunus, Shukor
format Article
author Mansur, Rusnam
Gusmanizar, Neni
Farrah Aini, Dahalan
Noor Azlina, Masdor
Siti Aqlima, Ahmad
Mohd Shukri, Shukor
Muhamad Akhmal Hakim, Roslan
Mohd Yunus, Shukor
author_sort Mansur, Rusnam
title Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
title_short Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
title_full Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
title_fullStr Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
title_full_unstemmed Isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia SP. Strain neni-11 in soils from west sumatera, Indonesia
title_sort isolation and characterization of a molybdenum-reducing and amide-degrading burkholderia sp. strain neni-11 in soils from west sumatera, indonesia
publisher Institute of Integrative Omics and Applied Biotechnology (IIOAB)
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41252
_version_ 1643799623723122688
score 13.209306

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