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Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site

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Main Authors: Mohd Yunus, Shukor, Farrah Aini, Dahalan, Jusoh, A. Z., Radzali, Muse, Nor Aripin, Shamaan, Mohd Arif, Syed
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: Triveni Enterprises 2016
Subjects:
Characterization
Diesel-degrading
Isolation
S. aureus
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41169
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spelling my.unimap-411692016-03-21T09:09:29Z Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site Mohd Yunus, Shukor Farrah Aini, Dahalan Jusoh, A. Z. Radzali, Muse Nor Aripin, Shamaan Mohd Arif, Syed farrahaini@unimap.edu.my yunus@biotech.upm.edu.my Characterization Diesel-degrading Isolation S. aureus Link to publisher's homepage at http://www.jeb.co.in A diesel-degrading bacterium has been isolated from a diesel-polluted site. The isolate was tentatively identified as Staphylococcus aureus strain DRY11 based on partial 16S rDNA molecular phylogeny and Biolog® GP microplate panels and Microlog® database. Isolate 11 showed an almost linear increase in cellular growth with respect to diesel concentrations with optimum growth occurring at 4% (v/v) diesel concentration. Optimization studies using different nitrogen sources showed that the best nitrogen source was potassium nitrite. Sodium nitrite was optimum at 1.2 g 1-1 and higher concentrations were strongly inhibitory to cellular growth. The optimal pH that supported growth of the bacterium was between 7.5 to 8.0 and the isolate exhibited optimal broad temperature supporting growth on diesel from 27 to 37°C. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 5 days of incubation. The characteristics of this bacterium suggest that it is suitable for bioremediation of diesel spills and pollutions in the tropics. 2016-03-21T09:07:07Z 2016-03-21T09:07:07Z 2009-01 Article Journal of Environmental Biology, vol.30 (1), 2009, pages 145-150 0254-8704 http://www.jeb.co.in/journal_issues/200901_jan09_spl/paper_23.pdf http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41169 en Triveni Enterprises
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Characterization
Diesel-degrading
Isolation
S. aureus
spellingShingle Characterization
Diesel-degrading
Isolation
S. aureus
Mohd Yunus, Shukor
Farrah Aini, Dahalan
Jusoh, A. Z.
Radzali, Muse
Nor Aripin, Shamaan
Mohd Arif, Syed
Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
description Link to publisher's homepage at http://www.jeb.co.in
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Mohd Yunus, Shukor
Farrah Aini, Dahalan
Jusoh, A. Z.
Radzali, Muse
Nor Aripin, Shamaan
Mohd Arif, Syed
format Article
author Mohd Yunus, Shukor
Farrah Aini, Dahalan
Jusoh, A. Z.
Radzali, Muse
Nor Aripin, Shamaan
Mohd Arif, Syed
author_sort Mohd Yunus, Shukor
title Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
title_short Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
title_full Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
title_fullStr Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
title_full_unstemmed Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
title_sort characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site
publisher Triveni Enterprises
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41169
_version_ 1643799598431469568
score 13.214268

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