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Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates

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Main Authors: Mohd Khalizan, Sabullah, Siti Aqlima, Ahmad, Mohd Yunus, Shukor, Nor Arifin, Shamaan, Ariff, Khalid, Azlan Jualang, Gansau, Farrah Aini, Dahalan, Mohd Rosni, Sulaiman
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: SPB Pharma Society 2016
Subjects:
Acetylcholinesterase
Carbamate
Organophosphate
Puntius javanicus
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41167
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spelling my.unimap-411672016-03-21T08:47:18Z Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates Mohd Khalizan, Sabullah Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Arifin, Shamaan Ariff, Khalid Azlan Jualang, Gansau Farrah Aini, Dahalan Mohd Rosni, Sulaiman farrahaini@unimap.edu.my rossulma@ums.edu.my Acetylcholinesterase Carbamate Organophosphate Puntius javanicus Link to publisher's homepage at http://jchps.com/ A new alternative local source of acetylcholinesterase (AChE) has been found to be sensitive towards several insecticides. AChE was purified from the brain tissue of Puntius javanicususing affinity chromatography (procainamide hydrochloride coupled with sephacryl 6B) with the total recovery of 40% at 8.48 purification fold. All carbamate (CB) insecticides tested at the concentration of 1 mg/L were capable of lowering AChE activity to less than 30%; Bendiocarp (18.80%), carbaryl (5.96%,), carbofuran (6.12%), methomyl (13.91%) and propoxur (15.851%). The P. javanicus AChE was found to be unaffected by several activated organophosphates (OP) such as acephate and dimethoate, while trichlorfon slightly inhibited the enzyme activity (p<0.05). Chlorpyrifos, diazinon, malathion and parathion lowered AChE activity to 43.02, 40.97, 37.11 and 46.72%, respectively. Pesticides that inhibit AChE activities more than 50% were further tested in different concentrations to determine the half maximal inhibitory concentration (IC50). Carbofuran and carbaryl showed the lowest IC50 value at 0.035 and 0.031 mg/L, respectively, as both showed no significant differences (p<0.05), followed by bendiocarp, propoxur, methomyl, malathion, diazinon, parathion and chlorpyrifos at 0.045, 0.076, 0.090, 0.063, 0.103, 0.151 and 0.202 mg/L, respectively. Based on these results, the sensitivity of AChE from brain P. javanicus brain tissue shows promise as an alternative biosensor for the detection of insecticides contamination. 2016-03-21T08:46:36Z 2016-03-21T08:46:36Z 2015-04-01 Article Journal of Chemical and Pharmaceutical Sciences, vol.8 (2), 2015, pages 348-353 0974-2115 http://jchps.com/past.html#v8is2 http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41167 en SPB Pharma Society
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Acetylcholinesterase
Carbamate
Organophosphate
Puntius javanicus
spellingShingle Acetylcholinesterase
Carbamate
Organophosphate
Puntius javanicus
Mohd Khalizan, Sabullah
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Arifin, Shamaan
Ariff, Khalid
Azlan Jualang, Gansau
Farrah Aini, Dahalan
Mohd Rosni, Sulaiman
Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
description Link to publisher's homepage at http://jchps.com/
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Mohd Khalizan, Sabullah
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Arifin, Shamaan
Ariff, Khalid
Azlan Jualang, Gansau
Farrah Aini, Dahalan
Mohd Rosni, Sulaiman
format Article
author Mohd Khalizan, Sabullah
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Arifin, Shamaan
Ariff, Khalid
Azlan Jualang, Gansau
Farrah Aini, Dahalan
Mohd Rosni, Sulaiman
author_sort Mohd Khalizan, Sabullah
title Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
title_short Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
title_full Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
title_fullStr Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
title_full_unstemmed Acetylcholinesterase from Puntius javanicus for the detection of carbamates and organophosphates
title_sort acetylcholinesterase from puntius javanicus for the detection of carbamates and organophosphates
publisher SPB Pharma Society
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41167
_version_ 1643799597834829824
score 13.214268

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