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Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals

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Main Authors: Nursabrina, Mohd Hayat, Nor Aripin, Shamaan, Mohd Yunus, Shukor, Mohd Khalizan, Sabullah, Mohd. Arif, Syed, Farrah Aini, Dahalan, Khalilalh, Abdul Khalil, Siti Aqlima, Ahmad
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: SPB Pharma Society 2016
Subjects:
Biomarker
Biomonitoring
Cholinesterase
Heavy metals
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41166
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spelling my.unimap-411662016-03-21T08:41:16Z Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals Nursabrina, Mohd Hayat Nor Aripin, Shamaan Mohd Yunus, Shukor Mohd Khalizan, Sabullah Mohd. Arif, Syed Farrah Aini, Dahalan Khalilalh, Abdul Khalil Siti Aqlima, Ahmad farrahaini@unimap.edu.my aqlima@upam.edu.my Biomarker Biomonitoring Cholinesterase Heavy metals Link to publisher's homepage at http://jchps.com/ Heavy metals are sometimes needed for nutrient uptake but only in low concentrations and the nervous system is the most susceptible to be affected. Cholinesterase (ChE) inhibition has been used extensively as a biomarker for heavy metals detection. In this study, the potential of ChE from Lates calcarifer brain as an alternative biosensor for heavy metals is evaluated. There are many Malaysian rivers such as Sungai Juru and Sungai Merbok that are greatly polluted by industrial effluents. Chronic exposure to heavy metals may cause nervous system disorders. Thus, a fast and simple biomonitoring technique will help in alerting government agencies and the public to such a threat. In this study, ChE from the brain of L. calcarifer (Asian seabass) was purified through ammonium sulphate precipitation and ion exchange chromatography. Enzyme recovery was 5.31% with a specific activity of 5.472 U mg-1. The Michaelis-Menten constant (Km) value was 0.3075 mM and a Vmax of 0.0304 μmol min-1 mg-1. ChE from L. calcarifer brain showed higher affinity to acetylthiocholine iodide (ATC) compared to butyrylcholinesterase (BTC) and propionylcholinesterase iodide (PTC). Maximum activity of ChE was detected when the enzyme was assayed at the temperature of 20-30°C and incubated in Tris-HCl buffer pH 8.0. Silver (Ag), arsenic (As), cadmium (Cd), chromium (Cr) and mercury (Hg) showed more than 50% inhibition amongst the 10 types of heavy metals that have been tested for inhibition study. These results can be used to further develop an alternative way to detect heavy metals that are low cost and give faster results compared to existing biosensor kits. 2016-03-21T08:40:18Z 2016-03-21T08:40:18Z 2015-04-01 Article Journal of Chemical and Pharmaceutical Sciences, vol. 8 (2), 2015, pages 376-381 0974-2115 http://jchps.com/past.html#v8is2 http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41166 en SPB Pharma Society
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biomarker
Biomonitoring
Cholinesterase
Heavy metals
spellingShingle Biomarker
Biomonitoring
Cholinesterase
Heavy metals
Nursabrina, Mohd Hayat
Nor Aripin, Shamaan
Mohd Yunus, Shukor
Mohd Khalizan, Sabullah
Mohd. Arif, Syed
Farrah Aini, Dahalan
Khalilalh, Abdul Khalil
Siti Aqlima, Ahmad
Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
description Link to publisher's homepage at http://jchps.com/
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Nursabrina, Mohd Hayat
Nor Aripin, Shamaan
Mohd Yunus, Shukor
Mohd Khalizan, Sabullah
Mohd. Arif, Syed
Farrah Aini, Dahalan
Khalilalh, Abdul Khalil
Siti Aqlima, Ahmad
format Article
author Nursabrina, Mohd Hayat
Nor Aripin, Shamaan
Mohd Yunus, Shukor
Mohd Khalizan, Sabullah
Mohd. Arif, Syed
Farrah Aini, Dahalan
Khalilalh, Abdul Khalil
Siti Aqlima, Ahmad
author_sort Nursabrina, Mohd Hayat
title Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
title_short Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
title_full Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
title_fullStr Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
title_full_unstemmed Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals
title_sort cholinesterase-based biosensor using lates calcarifer (asian seabass) brain for detection of heavy metals
publisher SPB Pharma Society
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41166
_version_ 1643799597491945472
score 13.214268

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