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The use of lates calcarifer as a biomarker for heavy metals detection

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Main Authors: Nursabrina, Mohd Hayat, Mohd Khalizan Halizan, Sabullah, Ariff, Khalid, Farrah Aini, Dahalan, Siti Aqlima, Ahmad, Mohd Yunus, Shukor, Nor Aripin, Shamaan, Mohd Arif, Syed
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: Springer Milan 2016
Subjects:
Biomarker
Biosensor
Cholinesterase
Heavy metals
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41114
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spelling my.unimap-411142016-03-09T04:03:42Z The use of lates calcarifer as a biomarker for heavy metals detection Nursabrina, Mohd Hayat Mohd Khalizan Halizan, Sabullah Ariff, Khalid Farrah Aini, Dahalan Siti Aqlima, Ahmad Mohd Yunus, Shukor Nor Aripin, Shamaan Mohd Arif, Syed farrahaini@unimap.edu.my Biomarker Biosensor Cholinesterase Heavy metals Link to publisher's homepage at http://link.springer.com Fish are ubiquitous organisms that have many features that designate their potential as a biomarker of heavy metals pollution. Thus, an investigation was done to detect the effect of heavy metals on cholinesterase (ChE) activity from Lates calcarifer organs which were gill and muscle. Ammonium sulphate precipitation was performed along with ion exchange chromatography to purify the enzyme. In the substrate specificity study, ChE from L. calcarifer gills was capable of breaking down acetylthiocholine iodide (ATC) at a faster rate compared to the other two synthetic substrates, which are butyrylthiocholine iodide (BTC) and propionylthiocholine iodide (PTC). In contrast, the muscle ChE has a higher affinity towards PTC. The maximum activity of ChE observed at the temperature ranging from 20 to 30 °C in Tris–HCl buffer pH 8. ChE from the two organs of L. calcarifer showed an inhibitive reaction towards heavy metals, but with different effects. ATC from gills showed 50 % inhibition by Cu, Hg and Pb, while PTC from muscle showed 50 % inhibition by Pb. The variation of inhibitory effect that was shown by ChE from L. calcarifer organs can be further studied in designing a biosensor kit that is sensitive towards heavy metal. 2016-03-09T04:01:39Z 2016-03-09T04:01:39Z 2016-01-21 Article Rendiconti Lincei, 2016, pages 1-10 1120-6349 1720-0776 (online) http://link.springer.com/article/10.1007/s12210-015-0501-7 http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41114 10.1007/s12210-015-0501-7 en Springer Milan
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biomarker
Biosensor
Cholinesterase
Heavy metals
spellingShingle Biomarker
Biosensor
Cholinesterase
Heavy metals
Nursabrina, Mohd Hayat
Mohd Khalizan Halizan, Sabullah
Ariff, Khalid
Farrah Aini, Dahalan
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Aripin, Shamaan
Mohd Arif, Syed
The use of lates calcarifer as a biomarker for heavy metals detection
description Link to publisher's homepage at http://link.springer.com
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Nursabrina, Mohd Hayat
Mohd Khalizan Halizan, Sabullah
Ariff, Khalid
Farrah Aini, Dahalan
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Aripin, Shamaan
Mohd Arif, Syed
format Article
author Nursabrina, Mohd Hayat
Mohd Khalizan Halizan, Sabullah
Ariff, Khalid
Farrah Aini, Dahalan
Siti Aqlima, Ahmad
Mohd Yunus, Shukor
Nor Aripin, Shamaan
Mohd Arif, Syed
author_sort Nursabrina, Mohd Hayat
title The use of lates calcarifer as a biomarker for heavy metals detection
title_short The use of lates calcarifer as a biomarker for heavy metals detection
title_full The use of lates calcarifer as a biomarker for heavy metals detection
title_fullStr The use of lates calcarifer as a biomarker for heavy metals detection
title_full_unstemmed The use of lates calcarifer as a biomarker for heavy metals detection
title_sort use of lates calcarifer as a biomarker for heavy metals detection
publisher Springer Milan
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41114
_version_ 1643799577597313024
score 13.211869

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