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Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation

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Bibliographic Details
Main Author: Pravinnaath, Kuppusamy
Other Authors: Zulkarnain Mohd Idris
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2015
Subjects:
Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38610
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spelling my.unimap-386102015-01-20T07:05:09Z Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation Pravinnaath, Kuppusamy Zulkarnain Mohd Idris Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Access is limited to UniMAP community. Phanerochaete chrysosporium known as white rot fungus has the ability to degrade the abundant aromatic polymer lignin. In this study, the extractions for antioxidant activity of Phanerochaete chrysosporium biomass extract were determined by using Central Composite Experimental Design. The extraction conditions studied were solid-to-solvent ratio, extraction pH and extraction time, while agitation speed, temperature and solvent concentration was set at the minimum value. All three parameters used give significant value which are then can be proceed for the optimization process. Folin-Ciocalteu reagents were used to determined the total phenolic content while 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging assay was used to analyze the antioxidant activity. From the result, there were no correlation found between total phenolic compounds and antioxidant activity. Phanerochaete chrysosporium biomass extract demonstrated as most potential free radical scavenging activity showing 82.80 % compares with ascorbic acid (Vitamin C) that showed 54.25 %. The optimization process meets the conditions at extraction pH 3.28, solid-tosolvent ratio 15.89 and extraction time 167.91, as the result showed the antioxidant activity is 81.98 % just slightly closed to the predicted value 82.27 %. 2015-01-20T07:05:09Z 2015-01-20T07:05:09Z 2013-05 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38610 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
spellingShingle Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
Pravinnaath, Kuppusamy
Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
description Access is limited to UniMAP community.
author2 Zulkarnain Mohd Idris
author_facet Zulkarnain Mohd Idris
Pravinnaath, Kuppusamy
format Learning Object
author Pravinnaath, Kuppusamy
author_sort Pravinnaath, Kuppusamy
title Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
title_short Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
title_full Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
title_fullStr Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
title_full_unstemmed Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
title_sort optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2015
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38610
_version_ 1643798801140416512
score 13.214268

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