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Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor

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Bibliographic Details
Main Author: Tan, Chee Kang
Other Authors: Dr. A.K.M Shafiqul Islam
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2015
Subjects:
Biosensor
Glucose oxidase (GOX) enzyme
Nanotubes
Carbon paste electrode
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465
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spelling my.unimap-384652015-01-11T08:30:20Z Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor Tan, Chee Kang Dr. A.K.M Shafiqul Islam Biosensor Glucose oxidase (GOX) enzyme Nanotubes Carbon paste electrode Access is limited to UniMAP community. A hybrid sol-gel carbon nanotubes composite has been developed and used for surface covalent bonding of enzyme for biosensing application. The biosensor was developed by covalent immobilization of glucose oxidase enzyme on sol gel modified electrode with ferrocene via carbodiimide reaction. The presence of amine groups provided by APTMOS in sol gel controlled the covalent bonding sites for enzyme. The experiment conditions such as amount of enzyme and pH in working buffer was studied by using Response Surface Methodology (RSM) through Central Composite Design (CCD) of Design Expert software. The biosensor showed maximum current response at 20 U/mL of immobilized glucose oxidase at the optimum pH value of working buffer of pH 7. The calibration curve of GOx biosensor was linear in the range of 0.1 mM to 3 mM of glucose concentration with a linear equation and correlation coefficient of 0.9873. The performance of GOx biosensor was evaluated based on selectivity toward the interferences and stability, the GOx biosensor exhibited high selectivity behavior and good stability. 2015-01-11T08:30:20Z 2015-01-11T08:30:20Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biosensor
Glucose oxidase (GOX) enzyme
Nanotubes
Carbon paste electrode
spellingShingle Biosensor
Glucose oxidase (GOX) enzyme
Nanotubes
Carbon paste electrode
Tan, Chee Kang
Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
description Access is limited to UniMAP community.
author2 Dr. A.K.M Shafiqul Islam
author_facet Dr. A.K.M Shafiqul Islam
Tan, Chee Kang
format Learning Object
author Tan, Chee Kang
author_sort Tan, Chee Kang
title Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
title_short Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
title_full Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
title_fullStr Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
title_full_unstemmed Immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
title_sort immobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensor
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2015
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465
_version_ 1643798767814574080
score 13.214268

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