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Development of protein extraction from agricultural waste as potential growth stimulator

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Main Author: Teoh, Seong Yee
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2015
Subjects:
Protein extraction
Soybean waste
Agricultural waste
Protein extractability
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38462
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spelling my.unimap-384622015-01-08T14:37:05Z Development of protein extraction from agricultural waste as potential growth stimulator Teoh, Seong Yee Protein extraction Soybean waste Agricultural waste Protein extractability Access is limited to UniMAP community. Soybean waste obtained during processing of soy products was found to be rich in protein and adequate in supporting the growth of microorganisms such as fungus Rhizopus oligosporus. The objective of this study was to optimize a process for extracting proteins from soybean waste and develop the protein extract as growth stimulator. The extraction process involved homogenizing the soybean waste in phosphate buffer solution with NaCl to dissolve and maximize the proteins solubility and centrifuging the water soluble fraction. In order to improve the protein extraction yield, the optimum pH buffer and the solid-to-liquid ratio were determined through optimization using response surface methodology. The results proved that the protein extraction step was effective and successfully enhanced the protein extraction yield. Two steps design of experiments were carried out and the best conditions for maximizing protein extraction yield were found to be homogenizing with phosphate buffer of pH 7 and solid-to-liquid ratio of 7 %w/v. The optimized protein extract was used to develop the agar culture medium along with glucose monohydrate which showed significant growth of fungus Rhizopus oligosporus and Phanerochaete chrysosporium indicating the soybean waste’s protein extract was a potential growth stimulator. 2015-01-08T14:37:05Z 2015-01-08T14:37:05Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38462 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Protein extraction
Soybean waste
Agricultural waste
Protein extractability
spellingShingle Protein extraction
Soybean waste
Agricultural waste
Protein extractability
Teoh, Seong Yee
Development of protein extraction from agricultural waste as potential growth stimulator
description Access is limited to UniMAP community.
format Learning Object
author Teoh, Seong Yee
author_facet Teoh, Seong Yee
author_sort Teoh, Seong Yee
title Development of protein extraction from agricultural waste as potential growth stimulator
title_short Development of protein extraction from agricultural waste as potential growth stimulator
title_full Development of protein extraction from agricultural waste as potential growth stimulator
title_fullStr Development of protein extraction from agricultural waste as potential growth stimulator
title_full_unstemmed Development of protein extraction from agricultural waste as potential growth stimulator
title_sort development of protein extraction from agricultural waste as potential growth stimulator
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2015
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38462
_version_ 1643798772935819264
score 13.214268

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