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Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass

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Bibliographic Details
Main Author: Tan, Vin Chzee
Other Authors: Zulkarnain Mohamed Idris
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2015
Subjects:
Natural antioxidant
Phanerochaete chrysosporium
Phenolic compound
Aqueous Two Phase System (ATPS)
Phenolic
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38459
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spelling my.unimap-384592015-01-08T14:22:24Z Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass Tan, Vin Chzee Zulkarnain Mohamed Idris Natural antioxidant Phanerochaete chrysosporium Phenolic compound Aqueous Two Phase System (ATPS) Phenolic Access is limited to UniMAP community. Natural antioxidants demand has increased globally due to the advantages and effect in various aspects, especially in pharmaceutical industry. Fungus Phanerochaete chrysosporium presents crude bioactive phenolics which act as antioxidant. The aim of this study was to develop an Aqueous Two Phase System (ATPS) to separate the phenolic compounds with high scavenging activity from Phanerochaete chrysosporium biomass. The main objective was to study the effect of salt concentration and pH on the partition of bioactive phenolics in solid-liquid ATPS. The second objective was to evaluate and compare the scavenging activity of phenolic extracted by ATPS with ascorbic acid. In order to extract the crude bioactive phenolics from the biomass of the fungus, a low cost and effective yet harmless to the environment ATP extraction technique was developed. The ATPS was developed by construct a phase diagram using appropriate concentration of the two phase forming components which in this case 80 % (w/w) of ethanol and 40 % (w/w) of K2HPO4. From the biphasic region indicated in the phase diagram, several points which give different composition of the components were selected to be test on the partition coefficient and recovery. The analytical result of total phenolic contents using Gallic acid as the standard was expressed in K and Rv. The best K (8.19) and Rv (97.81 %) was achieved by top phase of ATPS consisted of 50 % (w/v) ethanol and 20 % (w/v) K2HPO4 with 30 % (v/v) distilled water and 28 mg of biomass at pH 9.59. The capability of the phenolics in scavenging activity was tested by DPPH and the result was 81.77 % which considered high compared to the synthetic Ascorbic acid. 2015-01-08T14:22:24Z 2015-01-08T14:22:24Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38459 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Natural antioxidant
Phanerochaete chrysosporium
Phenolic compound
Aqueous Two Phase System (ATPS)
Phenolic
spellingShingle Natural antioxidant
Phanerochaete chrysosporium
Phenolic compound
Aqueous Two Phase System (ATPS)
Phenolic
Tan, Vin Chzee
Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
description Access is limited to UniMAP community.
author2 Zulkarnain Mohamed Idris
author_facet Zulkarnain Mohamed Idris
Tan, Vin Chzee
format Learning Object
author Tan, Vin Chzee
author_sort Tan, Vin Chzee
title Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
title_short Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
title_full Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
title_fullStr Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
title_full_unstemmed Development of Aqueous Two-Phase System (ATPS) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
title_sort development of aqueous two-phase system (atps) to separate crude bioactive phenolics from phanerochaete chrysosporium biomass
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2015
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/38459
_version_ 1643798772337082368
score 13.209306

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