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Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1

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Bibliographic Details
Main Author: Norazira, Ali
Other Authors: Khadijah Hanim Abdul Rahman
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2014
Subjects:
Bioprotein
Bioprotein production
Empty Fruit Bunch (EFB)
Aspergillus niger
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37638
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spelling my.unimap-376382014-11-05T06:18:01Z Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1 Norazira, Ali Khadijah Hanim Abdul Rahman Bioprotein Bioprotein production Empty Fruit Bunch (EFB) Aspergillus niger Access is limited to UniMAP community. Two steps optimization strategy based on stastisical experimental design was carried out to enhance bioprotein production by solid state fermentation utilizing empty fruit bunch (EFB) as substrate. Plackett-Burman design was employed first where 7 variables were studied for their influence in bioprotein production. NH4NO3, CuSO4.5H2O and MgSO4.7H2O were found to be most significant variables for improving bioprotein production. Central Composite Design (CCD) was employed to maximize the production of bioprotein. A mathematical model was developed to show the effects of each media component and their combinatorial interaction on bioprotein production. The optimal medium composition for maximum bioprotein production was are 0.70 % (v/w) of inorganic nitrogen source (NH4NO3), 0.06 % (v/w) of magnesium source (MgSO4.7H2O) and 0.23 % (v/w) of copper source (CuSO4.5H2O). Validation fermentation was carried out to validate the model. From the model validation process, predicted amount of protein concentration produced was 0.606598 mg/mL which is slightly higher than the experimental run in CCD which was 0.604 mg/mL. 2014-11-05T06:18:01Z 2014-11-05T06:18:01Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37638 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bioprotein
Bioprotein production
Empty Fruit Bunch (EFB)
Aspergillus niger
spellingShingle Bioprotein
Bioprotein production
Empty Fruit Bunch (EFB)
Aspergillus niger
Norazira, Ali
Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
description Access is limited to UniMAP community.
author2 Khadijah Hanim Abdul Rahman
author_facet Khadijah Hanim Abdul Rahman
Norazira, Ali
format Learning Object
author Norazira, Ali
author_sort Norazira, Ali
title Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
title_short Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
title_full Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
title_fullStr Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
title_full_unstemmed Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
title_sort optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus unimap aa-1
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2014
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37638
_version_ 1643798528365953024
score 13.18916

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