Cover Image

Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain

Access is limited to UniMAP community.

Saved in:
Bibliographic Details
Main Author: Nur Atiqah, Mad Arif
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2014
Subjects:
Sugarcane molasses
Polyhydroxyalkanoate (PHA)
Ralstonia eutropha
Fermentation
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimap-37626
record_format dspace
spelling my.unimap-376262014-11-05T02:18:49Z Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain Nur Atiqah, Mad Arif Mohamad Fahrurrazi Tompang Sugarcane molasses Polyhydroxyalkanoate (PHA) Ralstonia eutropha Fermentation Access is limited to UniMAP community. A study of polyhydroxyalkanoate (PHA) production was conducted using pretreatment of sugarcane molasses as main substrate to obtain low-cost medium for fermentation process. Ralstonia eutropha strain was used to produce polyhydroxyalkanoate in the nutrient limitation conditions. The study involved determination of significance interaction between different parameter which is sugarcane molasses, ammonium sulfate and urea using Central Composite Design in order to optimize the PHA concentration. Two-stage fermentation techniques were used to obtained high yield of PHA fermentation. First stage fermentation involved bacteria growth phase in a nutrient rich medium and second stage fermentation involved culturing bacteria strain in the limited nutrient medium. The fermentation process was incubated at fixed set point values of agitation speed, pH, fermentation period and temperature. The highest PHA composition recorded was 72.03 % at 2 % sugarcane molasses, 0.21 % ammonium sulfate and 1 % urea concentrations. 2014-11-05T02:18:49Z 2014-11-05T02:18:49Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Sugarcane molasses
Polyhydroxyalkanoate (PHA)
Ralstonia eutropha
Fermentation
spellingShingle Sugarcane molasses
Polyhydroxyalkanoate (PHA)
Ralstonia eutropha
Fermentation
Nur Atiqah, Mad Arif
Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Nur Atiqah, Mad Arif
format Learning Object
author Nur Atiqah, Mad Arif
author_sort Nur Atiqah, Mad Arif
title Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_short Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_full Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_fullStr Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_full_unstemmed Optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
title_sort optimization of media composition by using sugarcane molasses as substrate for production of polyhydroxyalkanote in ralstonia eutropha strain
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2014
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37626
_version_ 1643798525663772672
score 13.214268

Similar Items