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Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production

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Main Authors: Ku Syahidah, Ku Ismail, Sakamoto, Takatoshi, Hasunuma, Tomohisa, Kondo, Akihiko
Other Authors: kusyahidah@unimap.edu.my
Format: Article
Language:English
Published: Society for Industrial Microbiology and Biotechnology 2014
Subjects:
Bioethanol
Saccharomyces cerevisiae
Thermotolerant
Transcriptomics
Xylose
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/33471
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spelling my.unimap-334712014-04-07T14:33:53Z Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production Ku Syahidah, Ku Ismail Sakamoto, Takatoshi Hasunuma, Tomohisa Kondo, Akihiko kusyahidah@unimap.edu.my hasunuma@port.kobe-u.ac.jp akondo@kobe-u.ac.jp Bioethanol Saccharomyces cerevisiae Thermotolerant Transcriptomics Xylose Link to publisher's homepage at http://www.simbhq.org/ Agricultural residues comprising lignocellulosic materials are excellent sources of pentose sugar, which can be converted to ethanol as fuel. Ethanol production via consolidated bioprocessing requires a suitable microorganism to withstand the harsh fermentation environment of high temperature, high ethanol concentration, and exposure to inhibitors. We genetically enhanced an industrial Saccharomyces cerevisiae strain, sun049, enabling it to uptake xylose as the sole carbon source at high fermentation temperature. This strain was able to produce 13.9 g/l ethanol from 50 g/l xylose at 38 C. To better understand the xylose consumption ability during long-term, high-temperature conditions, we compared by transcriptomics two fermentation conditions: high temperature (38 C) and control temperature (30 C) during the first 12 h of fermentation. This is the first long-term, time-based transcriptomics approach, and it allowed us to discover the role of heat-responsive genes when xylose is the sole carbon source. The results suggest that genes related to amino acid, cell wall, and ribosomal protein synthesis are down-regulated under heat stress. To allow cell stability and continuous xylose uptake in order to produce ethanol, hexose transporter HXT5, heat shock proteins, ubiquitin proteins, and proteolysis were all induced at high temperature. We also speculate that the strong relationship between high temperature and increased xylitol accumulation represents the cell's mechanism to protect itself from heat degradation. 2014-04-07T14:33:53Z 2014-04-07T14:33:53Z 2013-09 Article Journal of Industrial Microbiology and Biotechnology, vol. 40(9), 2013, pages 1039-1050 1367-5435 http://link.springer.com/article/10.1007%2Fs10295-013-1293-3 http://dspace.unimap.edu.my:80/dspace/handle/123456789/33471 en Society for Industrial Microbiology and Biotechnology
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bioethanol
Saccharomyces cerevisiae
Thermotolerant
Transcriptomics
Xylose
spellingShingle Bioethanol
Saccharomyces cerevisiae
Thermotolerant
Transcriptomics
Xylose
Ku Syahidah, Ku Ismail
Sakamoto, Takatoshi
Hasunuma, Tomohisa
Kondo, Akihiko
Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
description Link to publisher's homepage at http://www.simbhq.org/
author2 kusyahidah@unimap.edu.my
author_facet kusyahidah@unimap.edu.my
Ku Syahidah, Ku Ismail
Sakamoto, Takatoshi
Hasunuma, Tomohisa
Kondo, Akihiko
format Article
author Ku Syahidah, Ku Ismail
Sakamoto, Takatoshi
Hasunuma, Tomohisa
Kondo, Akihiko
author_sort Ku Syahidah, Ku Ismail
title Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
title_short Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
title_full Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
title_fullStr Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
title_full_unstemmed Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
title_sort time-based comparative transcriptomics in engineered xylose-utilizing saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
publisher Society for Industrial Microbiology and Biotechnology
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/33471
_version_ 1643797194402168832
score 13.214268

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