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Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural

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Bibliographic Details
Main Authors: Hasunuma, Tomohisa, Ku Syahidah, Ku Ismail, Nambu, Yumiko, Kondo, Akihiko
Other Authors: hasunuma@port.kobe-u.ac.jp
Format: Article
Language:English
Published: Elsevier 2014
Subjects:
Furfural
TAL1
ADH1
Overexpression
Hemicellulosic hydrolysate
Xylose
Saccharomyces cerevisiae
Bioethanol
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/32778
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spelling my.unimap-327782014-03-15T07:42:49Z Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural Hasunuma, Tomohisa Ku Syahidah, Ku Ismail Nambu, Yumiko Kondo, Akihiko hasunuma@port.kobe-u.ac.jp kusyahidah@unimap.edu.my nambu@people.kobe-u.ac.jp akondo@kobe-u.ac.jp Furfural TAL1 ADH1 Overexpression Hemicellulosic hydrolysate Xylose Saccharomyces cerevisiae Bioethanol Link to publisher's homepage at http://www.elsevier.com Lignocellulosic biomass dedicated to bioethanol production usually contains pentoses and inhibitory compounds such as furfural that are not well tolerated by Saccharomyces cerevisiae. Thus, S. cerevisiae strains with the capability of utilizing both glucose and xylose in the presence of inhibitors such as furfural are very important in industrial ethanol production. Under the synergistic conditions of transaldolase (TAL) and alcohol dehydrogenase (ADH) overexpression, S. cerevisiae MT8-1X/TAL–ADH was able to produce 1.3-fold and 2.3-fold more ethanol in the presence of 70 mM furfural than a TAL-expressing strain and a control strain, respectively. We also tested the strains' ability by mimicking industrial ethanol production from hemicellulosic hydrolysate containing fermentation inhibitors, and ethanol production was further improved by 16% when using MT8-1X/TAL–ADH compared to the control strain. Transcript analysis further revealed that besides the pentose phosphate pathway genes TKL1 and TAL1, ADH7 was also upregulated in response to furfural stress, which resulted in higher ethanol production compared to the TAL-expressing strain. The improved capability of our modified strain was based on its capacity to more quickly reduce furfural in situ resulting in higher ethanol production. The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. 2014-03-15T07:42:49Z 2014-03-15T07:42:49Z 2014-02 Article Journal of Bioscience and Bioengineering, vol.117 (2), 2014, pages 165–169 1389-1723 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32778 http://www.sciencedirect.com/science/article/pii/S1389172313002697 http://dx.doi.org/10.1016/j.jbiosc.2013.07.007 en Elsevier
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Furfural
TAL1
ADH1
Overexpression
Hemicellulosic hydrolysate
Xylose
Saccharomyces cerevisiae
Bioethanol
spellingShingle Furfural
TAL1
ADH1
Overexpression
Hemicellulosic hydrolysate
Xylose
Saccharomyces cerevisiae
Bioethanol
Hasunuma, Tomohisa
Ku Syahidah, Ku Ismail
Nambu, Yumiko
Kondo, Akihiko
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
description Link to publisher's homepage at http://www.elsevier.com
author2 hasunuma@port.kobe-u.ac.jp
author_facet hasunuma@port.kobe-u.ac.jp
Hasunuma, Tomohisa
Ku Syahidah, Ku Ismail
Nambu, Yumiko
Kondo, Akihiko
format Article
author Hasunuma, Tomohisa
Ku Syahidah, Ku Ismail
Nambu, Yumiko
Kondo, Akihiko
author_sort Hasunuma, Tomohisa
title Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
title_short Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
title_full Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
title_fullStr Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
title_full_unstemmed Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
title_sort co-expression of tal1 and adh1 in recombinant xylose-fermenting saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
publisher Elsevier
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/32778
_version_ 1643796982654828544
score 13.222552

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