Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
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my.unimap-324952014-03-11T03:19:19Z Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays Penmatsa, Varun Ruslinda, A. Rahim Majid, Beidaghi Kawarada, Hiroshi Chunlei, Wang wangc@fiu.edu 3D carbon microarrays Direct amination Platelet-derived growth factor Fluorescence detection Link to publisher's homepage at http://www.elsevier.com/ The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5 pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins. 2014-03-11T03:19:19Z 2014-03-11T03:19:19Z 2013-01 Article Biosensors and bioelectronics, vol.39 (1), 2013, pages 118-123 0956-5663 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32495 http://www.sciencedirect.com/science/article/pii/S0956566312004149# http://dx.doi.org/10.1016/j.bios.2012.06.055 en Elsevier |
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3D carbon microarrays Direct amination Platelet-derived growth factor Fluorescence detection |
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3D carbon microarrays Direct amination Platelet-derived growth factor Fluorescence detection Penmatsa, Varun Ruslinda, A. Rahim Majid, Beidaghi Kawarada, Hiroshi Chunlei, Wang Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
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Link to publisher's homepage at http://www.elsevier.com/ |
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wangc@fiu.edu |
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wangc@fiu.edu Penmatsa, Varun Ruslinda, A. Rahim Majid, Beidaghi Kawarada, Hiroshi Chunlei, Wang |
format |
Article |
author |
Penmatsa, Varun Ruslinda, A. Rahim Majid, Beidaghi Kawarada, Hiroshi Chunlei, Wang |
author_sort |
Penmatsa, Varun |
title |
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
title_short |
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
title_full |
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
title_fullStr |
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
title_full_unstemmed |
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
title_sort |
platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays |
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Elsevier |
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2014 |
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http://dspace.unimap.edu.my:80/dspace/handle/123456789/32495 |
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1643796922870267904 |
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13.211869 |