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Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

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Main Authors: Penmatsa, Varun, Ruslinda, A. Rahim, Majid, Beidaghi, Kawarada, Hiroshi, Chunlei, Wang
Other Authors: wangc@fiu.edu
Format: Article
Language:English
Published: Elsevier 2014
Subjects:
3D carbon microarrays
Direct amination
Platelet-derived growth factor
Fluorescence detection
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/32495
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spelling my.unimap-324952014-03-11T03:19:19Z Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays Penmatsa, Varun Ruslinda, A. Rahim Majid, Beidaghi Kawarada, Hiroshi Chunlei, Wang wangc@fiu.edu 3D carbon microarrays Direct amination Platelet-derived growth factor Fluorescence detection Link to publisher's homepage at http://www.elsevier.com/ The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5 pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins. 2014-03-11T03:19:19Z 2014-03-11T03:19:19Z 2013-01 Article Biosensors and bioelectronics, vol.39 (1), 2013, pages 118-123 0956-5663 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32495 http://www.sciencedirect.com/science/article/pii/S0956566312004149# http://dx.doi.org/10.1016/j.bios.2012.06.055 en Elsevier
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic 3D carbon microarrays
Direct amination
Platelet-derived growth factor
Fluorescence detection
spellingShingle 3D carbon microarrays
Direct amination
Platelet-derived growth factor
Fluorescence detection
Penmatsa, Varun
Ruslinda, A. Rahim
Majid, Beidaghi
Kawarada, Hiroshi
Chunlei, Wang
Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
description Link to publisher's homepage at http://www.elsevier.com/
author2 wangc@fiu.edu
author_facet wangc@fiu.edu
Penmatsa, Varun
Ruslinda, A. Rahim
Majid, Beidaghi
Kawarada, Hiroshi
Chunlei, Wang
format Article
author Penmatsa, Varun
Ruslinda, A. Rahim
Majid, Beidaghi
Kawarada, Hiroshi
Chunlei, Wang
author_sort Penmatsa, Varun
title Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
title_short Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
title_full Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
title_fullStr Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
title_full_unstemmed Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
title_sort platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays
publisher Elsevier
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/32495
_version_ 1643796922870267904
score 13.211869

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