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Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose

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Main Authors: Ku Syahidah, Ku Ismail, Takatoshi, Sakamoto, Haruyo, Hatanaka, Tomohisa, Hasunuma, Akihiko, Kondo
Format: Article
Language:English
Published: Elsevier B.V. 2014
Subjects:
Bioethanol
Xylose
Thermotolerance
Microarray
Gene expression
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/32489
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spelling my.unimap-324892014-03-10T13:10:35Z Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose Ku Syahidah, Ku Ismail Takatoshi, Sakamoto Haruyo, Hatanaka Tomohisa, Hasunuma Akihiko, Kondo Bioethanol Xylose Thermotolerance Microarray Gene expression Link to publisher's homepage at http://www.elsevier.com/ Production of ethanol from xylose at high temperature would be an economical approach since it reduces risk of contamination and allows both the saccharification and fermentation steps in SSF to be running at elevated temperature. Eight recombinant xylose-utilizing Saccharomyces cerevisiae strains developed from industrial strains were constructed and subjected to high-temperature fermentation at 38 °C. The best performing strain was sun049T, which produced up to 15.2 g/L ethanol (63% of the theoretical production), followed by sun048T and sun588T, both with 14.1 g/L ethanol produced. Via transcriptomic analysis, expression profiling of the top three best ethanol producing strains compared to a negative control strain, sun473T, led to the discovery of genes in common that were regulated in the same direction. Identification of the 20 most highly up-regulated and the 20 most highly down-regulated genes indicated that the cells regulate their central metabolism and maintain the integrity of the cell walls in response to high temperature. We also speculate that cross-protection in the cells occurs, allowing them to maintain ethanol production at higher concentration under heat stress than the negative controls. This report provides further transcriptomics information in the interest of producing a robust microorganism for high-temperature ethanol production utilizing xylose. 2014-03-10T13:10:35Z 2014-03-10T13:10:35Z 2013-01 Article Journal of Biotechnology, vol.163 (1), 2013, pages 50-60 0168-1656 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32489 http://www.sciencedirect.com/science/article/pii/S016816561200692X en Elsevier B.V.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bioethanol
Xylose
Thermotolerance
Microarray
Gene expression
spellingShingle Bioethanol
Xylose
Thermotolerance
Microarray
Gene expression
Ku Syahidah, Ku Ismail
Takatoshi, Sakamoto
Haruyo, Hatanaka
Tomohisa, Hasunuma
Akihiko, Kondo
Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
description Link to publisher's homepage at http://www.elsevier.com/
format Article
author Ku Syahidah, Ku Ismail
Takatoshi, Sakamoto
Haruyo, Hatanaka
Tomohisa, Hasunuma
Akihiko, Kondo
author_facet Ku Syahidah, Ku Ismail
Takatoshi, Sakamoto
Haruyo, Hatanaka
Tomohisa, Hasunuma
Akihiko, Kondo
author_sort Ku Syahidah, Ku Ismail
title Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
title_short Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
title_full Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
title_fullStr Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
title_full_unstemmed Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
title_sort gene expression cross-profiling in genetically modified industrial saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
publisher Elsevier B.V.
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/32489
_version_ 1643796921073008640
score 13.214268

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