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Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

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Main Authors: Ruslinda, Abdul Rahim, Dr., Ishiyama, Y., Wang, Xianfen, Kobayashi, T., Hiroshi, Kawarada, Prof. Dr.
Other Authors: ruslinda@toki.waseda.jp
Format: Article
Language:English
Published: The Electrochemical Society 2014
Subjects:
Adenosine triphosphate (ATP)
Platelet derived growth factor (PDGF)
Diamond
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/31516
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spelling my.unimap-315162014-01-27T07:53:04Z Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface Ruslinda, Abdul Rahim, Dr. Ishiyama, Y. Wang, Xianfen Kobayashi, T. Hiroshi, Kawarada, Prof. Dr. ruslinda@toki.waseda.jp ruslinda@unimap.edu.my kawarada@waseda.jp Adenosine triphosphate (ATP) Platelet derived growth factor (PDGF) Diamond Link to publisher's homepage at http://www.electrochem.org/ An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding. 2014-01-27T07:53:04Z 2014-01-27T07:53:04Z 2012 Article Journal of the Electrochemical Society, vol. 159(5), 2012, pages J182-J187 0013-4651 http://jes.ecsdl.org/content/159/5/J182 http://dspace.unimap.edu.my:80/dspace/handle/123456789/31516 en The Electrochemical Society
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Adenosine triphosphate (ATP)
Platelet derived growth factor (PDGF)
Diamond
spellingShingle Adenosine triphosphate (ATP)
Platelet derived growth factor (PDGF)
Diamond
Ruslinda, Abdul Rahim, Dr.
Ishiyama, Y.
Wang, Xianfen
Kobayashi, T.
Hiroshi, Kawarada, Prof. Dr.
Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
description Link to publisher's homepage at http://www.electrochem.org/
author2 ruslinda@toki.waseda.jp
author_facet ruslinda@toki.waseda.jp
Ruslinda, Abdul Rahim, Dr.
Ishiyama, Y.
Wang, Xianfen
Kobayashi, T.
Hiroshi, Kawarada, Prof. Dr.
format Article
author Ruslinda, Abdul Rahim, Dr.
Ishiyama, Y.
Wang, Xianfen
Kobayashi, T.
Hiroshi, Kawarada, Prof. Dr.
author_sort Ruslinda, Abdul Rahim, Dr.
title Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
title_short Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
title_full Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
title_fullStr Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
title_full_unstemmed Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface
title_sort fluorescence-signaling aptasensor for atp and pdgf detection on functionalized diamond surface
publisher The Electrochemical Society
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/31516
_version_ 1643796579845406720
score 13.214268

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