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Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture

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Bibliographic Details
Main Author: Hanna, Hashim
Other Authors: Dr. Zarina Zakaria
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Tissue culture
Stevia rebaudiana
Chemical sterilization agent
Sodium hypochloride (NaOCl)
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27736
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spelling my.unimap-277362013-08-27T08:41:40Z Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture Hanna, Hashim Dr. Zarina Zakaria Tissue culture Stevia rebaudiana Chemical sterilization agent Sodium hypochloride (NaOCl) Access is limited to UniMAP community. This study was to distinguish the efficiency of using sodium hypochloride, (NaOCl) in sterilization of explant Stevia rebaudiana in tissue culture technique. Sterilization technique is ought to be done for the successful in growth of the tissue culture especially Stevia rebaudiana which is the type of plant to be studied. The tenacity of sterilization technique was to diminish the risk that the microorganisms sustain on the cultures. NaOCl is use as modification on using mercury chloride, (HgCl2) which was very harmful. The plant part used was leaf (1 cm) and culture in Murashige & Skoog media. By determining the optimal concentration and time immersed of the NaOCl to be used with existence of ethanol solution, the optimum solution attained was 0.70 % of NaOCl concentration with time immersed of 20 minutes and plus existence of ethanol. 2013-08-27T08:41:40Z 2013-08-27T08:41:40Z 2012-06 Learning Object http://hdl.handle.net/123456789/27736 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Tissue culture
Stevia rebaudiana
Chemical sterilization agent
Sodium hypochloride (NaOCl)
spellingShingle Tissue culture
Stevia rebaudiana
Chemical sterilization agent
Sodium hypochloride (NaOCl)
Hanna, Hashim
Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
description Access is limited to UniMAP community.
author2 Dr. Zarina Zakaria
author_facet Dr. Zarina Zakaria
Hanna, Hashim
format Learning Object
author Hanna, Hashim
author_sort Hanna, Hashim
title Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
title_short Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
title_full Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
title_fullStr Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
title_full_unstemmed Optimization of chemical sterilization agent in Stevia rebaudiana tissue culture
title_sort optimization of chemical sterilization agent in stevia rebaudiana tissue culture
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27736
_version_ 1643795391517294592
score 13.214268

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