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Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics

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Main Author: Chen, Hui Wen
Other Authors: Zulkarnain Mohd Idris
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
Basidiomycetes
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27734
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spelling my.unimap-277342013-08-27T08:35:11Z Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics Chen, Hui Wen Zulkarnain Mohd Idris Antioxidant Phanerochaete chrysosporium Bioactive phenolics White rot fungus Basidiomycetes Access is limited to UniMAP community. Phanerochaete chrysosporium or known as white rot fungus has the major ability in lignin-degradable. However, antioxidant activities of the extracts from Phanerochaete chrysosporium biomass are poorly reported. In this study, the extraction conditions for antioxidant activity of Phanerochaete chrysosporium biomass extracts were determined by using Plackett-Burman experimental design. The extraction conditions studied were solid-solvent ratio, solvent concentration, agitation speed, extraction pH, temperature, and time. Positive effect variables such as solid-solvent ratio, pH, and time were found to be 24.19, 23.57, and 2.82 respectively. On contrary, agitation speed, solvent concentration and temperature had negatively effects on antioxidant activity at -0.45, -13.73, and -18.42 respectively. Folin-Ciocalteu reagents were used to determine the total phenolic compounds while 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay was used to analyze the antioxidant activity. From the results, there were no correlation between total phenolic compounds and antioxidant activity. But Phanerochaete chrysosporium biomass extract still demonstrated antioxidant activity where the most potent radical scavenging activity showing 84.21±0.01% compares with butylated hydroxytoluene (BHT) that showed 84.5 ±0.01%. 2013-08-27T08:35:11Z 2013-08-27T08:35:11Z 2012-06 Learning Object http://hdl.handle.net/123456789/27734 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
Basidiomycetes
spellingShingle Antioxidant
Phanerochaete chrysosporium
Bioactive phenolics
White rot fungus
Basidiomycetes
Chen, Hui Wen
Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
description Access is limited to UniMAP community.
author2 Zulkarnain Mohd Idris
author_facet Zulkarnain Mohd Idris
Chen, Hui Wen
format Learning Object
author Chen, Hui Wen
author_sort Chen, Hui Wen
title Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_short Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_fullStr Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full_unstemmed Effects of extraction conditions on antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_sort effects of extraction conditions on antioxidant activity of phanerochaete chrysosporium bioactive phenolics
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27734
_version_ 1643795390877663232
score 13.222552

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