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Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation

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Bibliographic Details
Main Author: Nor’izzah, Zainuddin
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Lipase
Oil palm pressed fiber (OPPF)
Aspergillus niger
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27672
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spelling my.unimap-276722013-08-26T09:13:32Z Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation Nor’izzah, Zainuddin Mohamad Fahrurrazi Tompang Lipase Oil palm pressed fiber (OPPF) Aspergillus niger Access is limited to UniMAP community. Lipase is an enzyme which capable to be catalyst in the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Oil palm pressed fiber (OPPF) is an agricultural waste know as mesocarp fiber which recovered from pressed palm oil and based on dry weight basis, approximately it contains of 40 % cellulose, 24 % pentosan, 21 % lignin and 5 % ash. The production of extracellular lipase from OPPF using Aspergillus niger by solid state fermentation (SSF) was studied. The main target in this study is to produce high yield of lipase activity from OPPF based on media composition which is inducer, carbon and nitrogen sources. The optimized media composition consisted of OPPF of size 500 μm, 70 % of moisture content at 32 °C. In addition, it is desired to determine the effect of interaction between parameter, olive oil as inducer (0.5 % – 1.5 % v/v), sucrose as carbon source (0.5 % – 1.5 % w/v) and peptone as nitrogen source (0.1 % – 0.3 % w/v). Optimization of media composition was done by using Response Surface Methodology (RSM): Central Composite Design (CCD). In the optimization process, the design contains of a total of 17 experimental sample runs involving replications of central points. The optimization of lipase production by Aspergillus niger using OPPF as solid substrates shown a maximum production of about 0.3763 U/mL of lipase activity which obtained from experimental run which contains of 0.37 % (v/v) of olive oil, 0.20 % (w/v) of sucrose and 0.04 % (w/v) of peptone in the fermentation medium. 2013-08-26T09:13:32Z 2013-08-26T09:13:32Z 2012-06 Learning Object http://hdl.handle.net/123456789/27672 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lipase
Oil palm pressed fiber (OPPF)
Aspergillus niger
spellingShingle Lipase
Oil palm pressed fiber (OPPF)
Aspergillus niger
Nor’izzah, Zainuddin
Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Nor’izzah, Zainuddin
format Learning Object
author Nor’izzah, Zainuddin
author_sort Nor’izzah, Zainuddin
title Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
title_short Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
title_full Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
title_fullStr Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
title_full_unstemmed Optimization of media composition of lipase production from oil palm press fiber (OPPF) by solid state fermentation
title_sort optimization of media composition of lipase production from oil palm press fiber (oppf) by solid state fermentation
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27672
_version_ 1643795449986940928
score 13.160551

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