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Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation

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Main Author: Siti Nadherah, Mohamad
Other Authors: Syazni Zainul Kamal
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Chicken feather
Keratinolytic microorganisms
Protein hydrolysate
Aspergillus terreus
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27671
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spelling my.unimap-276712013-08-26T08:50:35Z Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation Siti Nadherah, Mohamad Syazni Zainul Kamal Chicken feather Keratinolytic microorganisms Protein hydrolysate Aspergillus terreus Access is limited to UniMAP community. Feathers are by-product waste of poultry processing plant and in this research the protein hydrolysate production was investigated. Culture medium for protein hydrolysate production from feather by Aspergillus terreus UniMAP AA-1 was optimised. Effect of culture conditions on protein hydrolysate were tested and optimal results were obtained with a culture volume of 100 mL, culture pH value was 7.5, culture temperature was 35 °C and inoculums size of spore suspension was 2.5x108. To improve protein hydrolysate yield, statistically based experimental designs were applied to optimize the culture medium. 2-level factorial design experiments showed that from three parameters involved which was feather concentration, concentration yeast extract and concentration of K2HPO4, only feather concentration and concentration of K2HPO4 were the most significant factors affecting protein hydrolysate production. Furthermore, central composite design experiments indicated that the optimal feather concentration and concentration of K2HPO4 were 1.75 mg/mL and 0.1 mg/mL. In order to obtain the optimal protein hydrolysate concentration at 0.669 mg/mL, optimised fermentation mediums (mg/mL) such as KH2PO4 concentration, MgSO4 concentration and NaCl concentration were used at value of 0.8 mg/mL, 0.2 mg/mL and 1.0 mg/mL. Feather was degraded during submerged cultivating. The keratinous waste can be biologically degraded by enzymes or the microbe itself to form useful products. 2013-08-26T08:50:35Z 2013-08-26T08:50:35Z 2012-06 Learning Object http://hdl.handle.net/123456789/27671 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Chicken feather
Keratinolytic microorganisms
Protein hydrolysate
Aspergillus terreus
spellingShingle Chicken feather
Keratinolytic microorganisms
Protein hydrolysate
Aspergillus terreus
Siti Nadherah, Mohamad
Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
description Access is limited to UniMAP community.
author2 Syazni Zainul Kamal
author_facet Syazni Zainul Kamal
Siti Nadherah, Mohamad
format Learning Object
author Siti Nadherah, Mohamad
author_sort Siti Nadherah, Mohamad
title Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
title_short Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
title_full Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
title_fullStr Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
title_full_unstemmed Chemical parameter optimization of feather protein hydrolysate production by Aspergillus terreus UniMAP AA-1 using submerged fermentation
title_sort chemical parameter optimization of feather protein hydrolysate production by aspergillus terreus unimap aa-1 using submerged fermentation
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27671
_version_ 1643795449691242496
score 13.214268

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