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Optimization of process condition for bioprotein production in shake flask study

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Bibliographic Details
Main Author: Nur Azreen, Ahmad Puad
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Bioprotein
Shake flask study
Phanerochaete chrysosporium
White rot fungus
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27668
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spelling my.unimap-276682013-08-27T03:07:13Z Optimization of process condition for bioprotein production in shake flask study Nur Azreen, Ahmad Puad Mohamad Fahrurrazi Tompang Bioprotein Shake flask study Phanerochaete chrysosporium White rot fungus Access is limited to UniMAP community. Bioprotein is a protein that is cultivated from many types of microorganisms through fermentation process, either solid state fermentation or liquid state fermentation. The microorganism used was white rot fungus, Phanerochaete chrysosporium. The aim of this research is to optimize the process condition in order to produce high biomass of bioprotein in shake flask study. In addition, it is desired to study the effect of the interaction between the parameters, agitation speed, inoculum size and the initial pH. The research was done by using Central Composite Design (CCD) under Response Surface Methodology (RSM). Based on the screening of fermentation period, six days of incubation period was considered as the optimum time for submerged fermentation. In the optimization process, the design contains a total of 17 experimental runs at three level systems. From the analysis of variance (ANOVA) using quadratic polynomial equation, maximum bioprotein production and total protein content of obtained was 4.698g/L and 5.3345 mg/ml respectively by using 150 rpm, with 5.5% inoculum size at pH 5. The validation of model was done and showed that the model are valid as 4.469g/L bioprotein production and 4.5735 mg/mL of total protein respectively are obtained from the validation experiment. 2013-08-26T08:10:46Z 2013-08-26T08:10:46Z 2012-06 Learning Object http://hdl.handle.net/123456789/27668 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bioprotein
Shake flask study
Phanerochaete chrysosporium
White rot fungus
spellingShingle Bioprotein
Shake flask study
Phanerochaete chrysosporium
White rot fungus
Nur Azreen, Ahmad Puad
Optimization of process condition for bioprotein production in shake flask study
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Nur Azreen, Ahmad Puad
format Learning Object
author Nur Azreen, Ahmad Puad
author_sort Nur Azreen, Ahmad Puad
title Optimization of process condition for bioprotein production in shake flask study
title_short Optimization of process condition for bioprotein production in shake flask study
title_full Optimization of process condition for bioprotein production in shake flask study
title_fullStr Optimization of process condition for bioprotein production in shake flask study
title_full_unstemmed Optimization of process condition for bioprotein production in shake flask study
title_sort optimization of process condition for bioprotein production in shake flask study
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27668
_version_ 1643795448796807168
score 13.214268

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