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Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse

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Bibliographic Details
Main Author: Muhammad Lokman, Muhammad Ramdan
Other Authors: Syazni Zainul Kamal
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Cellulose
Aspergillus niger
Sugarcane bagasse
Cellulase enzyme
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27664
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spelling my.unimap-276642013-08-26T07:47:30Z Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse Muhammad Lokman, Muhammad Ramdan Syazni Zainul Kamal Cellulose Aspergillus niger Sugarcane bagasse Cellulase enzyme Access is limited to UniMAP community. Cellulase can be produced using fungi such as Aspergillus niger on solid state fermentation (SSF). But, the problem is the cost of producing the enzyme is expensive. The growing approaches to reduce the cost of cellulases production can be achieved via using of cheap, renewable agriculture wastes which consider an excellent carbon source for microbial enzymes production and also looking for microorganism having the ability to hyper-production of cellulases. Therefore, considering the advantages of SSF, the optimal chemical condition for the production of cellulase enzyme by Apergillus niger from solid-state fermentation of sugarcane bagasse is investigated. Three parameters was chosen to be screened and optimized using Design Expert Software (DOE). The parameters are amount of substrate (C-source), concentration of yeast extract (N-source) and concentration of Potassium di-Hydrogen Phosphate (KH2PO4) (Phosphate source). After screening, the parameter for N-source was not significant to the model. Thus, parameters C-source and Phosphate source was optimize. The optimum point predictions using DOE are 7.20 g for the amount of substrate and 3.6 g/L of KH2PO4 which resulted in enzyme activity of 0.356562 IU/mL. 2013-08-26T07:47:30Z 2013-08-26T07:47:30Z 2012-06 Learning Object http://hdl.handle.net/123456789/27664 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Cellulose
Aspergillus niger
Sugarcane bagasse
Cellulase enzyme
spellingShingle Cellulose
Aspergillus niger
Sugarcane bagasse
Cellulase enzyme
Muhammad Lokman, Muhammad Ramdan
Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
description Access is limited to UniMAP community.
author2 Syazni Zainul Kamal
author_facet Syazni Zainul Kamal
Muhammad Lokman, Muhammad Ramdan
format Learning Object
author Muhammad Lokman, Muhammad Ramdan
author_sort Muhammad Lokman, Muhammad Ramdan
title Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_short Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_full Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_fullStr Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_full_unstemmed Chemical parameter optimization of cellulase production by Aspergillus niger on solid state fermentation of sugarcane bagasse
title_sort chemical parameter optimization of cellulase production by aspergillus niger on solid state fermentation of sugarcane bagasse
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27664
_version_ 1643795385299238912
score 13.214268

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