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Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis

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Bibliographic Details
Main Authors: Huzairy, Hassan, Khairiah, Abdul Karim
Other Authors: chkhairiah@eng.usm.my
Format: Article
Language:English
Published: Chulalongkorn University, Faculty of Engineering 2013
Subjects:
Agricultural by-products
Alpha-amylase
Bacillus subtilis
Solid state fermentation
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/26551
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spelling my.unimap-265512013-07-10T04:58:47Z Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis Huzairy, Hassan Khairiah, Abdul Karim chkhairiah@eng.usm.my Agricultural by-products Alpha-amylase Bacillus subtilis Solid state fermentation Link to publisher's homepage at http://engj.org/ The production of alpha-amylase by Bacillus subtilis was investigated under different cultivation conditions on two different solid substrates as the support, i.e. oil palm empty fruit bunch (OPEFB) fibers and rice straw using solid state fermentation (SSF) process. Both solid substrates were first pre-treated using NaOH to remove lignin and silica and to break down the hemicellulose structures. The influences of incubation time, incubation temperature and the additional carbon and nitrogen sources on the production of alpha-amylase by B. subtilis were investigated. After 48 h of incubation, the highest enzyme activities were obtained at 39.9 U/g and 23.5 U/g for rice straw and OPEFB fibers, respectively. The optimum temperature for alpha-amylase production was observed at 45°C for the OPEFB fibers and 55°C for the rice straw. Among the defined carbohydrates, the addition of glucose (0.02 g/g dry substrate) has significantly improved the production of alpha-amylase. Similar effect was observed when yeast extract (0.01 g/g dry substrate) was supplemented as the additional nitrogen source. Rice straw appeared to be the best solid substrate in producing alpha-amylase (276 U/g) in SSF under these optimum cultivation conditions. 2013-07-10T04:58:47Z 2013-07-10T04:58:47Z 2012 Article Engineering Journal, vol. 16(5), 2012, pages 177-185 0125-8281 http://www.engj.org/index.php/ej/article/view/266/235 http://hdl.handle.net/123456789/26551 en Chulalongkorn University, Faculty of Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Agricultural by-products
Alpha-amylase
Bacillus subtilis
Solid state fermentation
spellingShingle Agricultural by-products
Alpha-amylase
Bacillus subtilis
Solid state fermentation
Huzairy, Hassan
Khairiah, Abdul Karim
Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
description Link to publisher's homepage at http://engj.org/
author2 chkhairiah@eng.usm.my
author_facet chkhairiah@eng.usm.my
Huzairy, Hassan
Khairiah, Abdul Karim
format Article
author Huzairy, Hassan
Khairiah, Abdul Karim
author_sort Huzairy, Hassan
title Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
title_short Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
title_full Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
title_fullStr Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
title_full_unstemmed Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis
title_sort utilization of agricultural by-products for alpha-amylase production under solid state fermentation by bacillus subtilis
publisher Chulalongkorn University, Faculty of Engineering
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/26551
_version_ 1643794982152175616
score 13.214268

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