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Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers

Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor.

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Bibliographic Details
Main Authors: Mohd Azlan, Mohd Ishak, Zarina, Zakaria, Abd Hamid, Khalid, Suhana, Zakaria
Format: Working Paper
Language:English
Published: Universiti Teknologi MARA (UiTM) 2008
Subjects:
Essences and essential oils
Plants > Analysis
Citrus grandis
Gas chromatography
Fragrance research
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/2639
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spelling my.unimap-26392009-12-31T04:09:26Z Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers Mohd Azlan, Mohd Ishak Zarina, Zakaria Abd Hamid, Khalid Suhana, Zakaria Essences and essential oils Plants -- Analysis Citrus grandis Gas chromatography Fragrance research Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor. Citrus grandis or pomelo flowers possess a strong floral-, jasmine- and orange-like fragrance. In this study, various sampling techniques such as hydrodistillation, Soxhlet extraction and Solid Phase Micro extraction (SPME) were used to compare the extraction capability of the fragrance from pomelo flowers. Gas Chromatograph - Mass Spectrometry (GCMS) was then used to identify the extracted components. Results thus far showed that, limonene, linalool, ocimene, naphthalene and caryophyllene were the major compounds detected. In the Soxhlet extraction method, limonene was a major compound detected on extracts of blossoms and buds with percent concentrations of 4.4 and 9.3%, respectively. On the contrary, analysis on extracts of both flower components via the hydrodistillation method was identified linalool as the major compound with percent concentrations of 8.3 and 2.7%, respectively. In the SPME method, however, the detection of limonene in both flower components was found to be the most efficient with the peak area of ca. 13 and 62%, respectively. In this method, three different types of coating fibres were evaluated and 100 µm polydimethylsiloxane coated fibre showed a high efficiency detection of fragrance compounds from pomelo flowers. Analysis of buds showed that, limonene attained the highest relative abundance followed by ocimene, caryophyllene and naphthalene. In tissue culture procedure, all six different parts of cultured explants namely petal, sepal, style, ovary, pistil and cup base showed a capability to form callus as early as day 5. Style and pistil were the best explants to form callus on Murashige and Skoog media which supplemented with 0.05 mg/l and 0.01 mg/l BAP, respectively. 2008-10-29T07:38:17Z 2008-10-29T07:38:17Z 2007-07-05 Working Paper http://hdl.handle.net/123456789/2639 en Proceedings of the Conference on Scientific & Social Research 2006/2007 (CSSR 06/07) Universiti Teknologi MARA (UiTM)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Essences and essential oils
Plants -- Analysis
Citrus grandis
Gas chromatography
Fragrance research
spellingShingle Essences and essential oils
Plants -- Analysis
Citrus grandis
Gas chromatography
Fragrance research
Mohd Azlan, Mohd Ishak
Zarina, Zakaria
Abd Hamid, Khalid
Suhana, Zakaria
Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
description Organized by Institute of Research, Development and Commercialisation (IRDC), UiTM, 3rd - 5th July 2007 at Sunway Lagoon Resort Hotel, Selangor.
format Working Paper
author Mohd Azlan, Mohd Ishak
Zarina, Zakaria
Abd Hamid, Khalid
Suhana, Zakaria
author_facet Mohd Azlan, Mohd Ishak
Zarina, Zakaria
Abd Hamid, Khalid
Suhana, Zakaria
author_sort Mohd Azlan, Mohd Ishak
title Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
title_short Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
title_full Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
title_fullStr Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
title_full_unstemmed Characterisation of fragrance major compouds in intact plant and in vitro cultures of Citrus grandis (Osbeck) flowers
title_sort characterisation of fragrance major compouds in intact plant and in vitro cultures of citrus grandis (osbeck) flowers
publisher Universiti Teknologi MARA (UiTM)
publishDate 2008
url http://dspace.unimap.edu.my/xmlui/handle/123456789/2639
_version_ 1643787705769787392
score 13.214268

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