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Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities

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Main Authors: Mohd Farhan, Abd Razak, Abd. Razak, Shaari, Pin, Kar Yong, Luqman Chuah, Abdullah, Prof. Dr.
Other Authors: mfarhan@frim.gov.m y
Format: Article
Language:English
Published: Forest Research Institute Malaysia 2013
Subjects:
2-diphenyl-1-picrylhydrazyl (DPPH)
Methanolic extract
Open environment
Shade treatment
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/26050
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spelling my.unimap-260502013-06-25T02:43:53Z Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities Mohd Farhan, Abd Razak Abd. Razak, Shaari Pin, Kar Yong Luqman Chuah, Abdullah, Prof. Dr. mfarhan@frim.gov.m y 2-diphenyl-1-picrylhydrazyl (DPPH) Methanolic extract Open environment Shade treatment Link to publisher's homepage at http://www.frim.gov.my/ Orthosiphon stamineus is an important medicinal plant in South-East Asia. Although already widely cultivated, the influence of growing conditions, especially light intensity, on the antioxidant property of O. stamineus is still unknown. Thus, the objective of this study was to determine the antioxidant activity and phenolic content of different plant parts of O. stamineus and to propose the best environment condition for its cultivation. The leaves, stems and roots of O. stamineus treated in open environment were extracted with methanol for 4 hours in a shaking water bath at 40°C. Total phenolic content (TPC) and antioxidant activity were determined using Folin-Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods respectively. In the first part of the experiment, only seedlings grown in the open environment were used. TPC was higher in leaves (230 mg gallic acid g -1 dry weight) compared with stems and roots (160 and 140 mg gallic acid g -1 dry weight respectively). However, antioxidant activity was lower in leaves (78.43%) than stems (80.66%) and roots (80.26%). By selecting leaves as the main part to study, O. stamineus grown under shaded treatment (50 and 70% shaded) as well as open environment were harvested to determine their TPC and antioxidant activities. It was observed that TPC in O. stamineus leaves grown in open environment was highest (230 mg gallic acid g -1 dry weight) compared with shade-grown seedlings. Similarly, antioxidant activity was also highest in seedlings grown in open environment (80.02%) compared with in shade (50% = 77.72%, 70% = 74.37%) 2013-06-25T02:43:53Z 2013-06-25T02:43:53Z 2012-04 Article Journal of Tropical Forest Science, vol. 24(2), 2012, pages 173-177 0128-1283 (P) 0128-1283 (E) http://www.frim.gov.my/v1/JTFSOnline/jtfs/v24n2/173-177.pdf http://hdl.handle.net/123456789/26050 en Forest Research Institute Malaysia
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic 2-diphenyl-1-picrylhydrazyl (DPPH)
Methanolic extract
Open environment
Shade treatment
spellingShingle 2-diphenyl-1-picrylhydrazyl (DPPH)
Methanolic extract
Open environment
Shade treatment
Mohd Farhan, Abd Razak
Abd. Razak, Shaari
Pin, Kar Yong
Luqman Chuah, Abdullah, Prof. Dr.
Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
description Link to publisher's homepage at http://www.frim.gov.my/
author2 mfarhan@frim.gov.m y
author_facet mfarhan@frim.gov.m y
Mohd Farhan, Abd Razak
Abd. Razak, Shaari
Pin, Kar Yong
Luqman Chuah, Abdullah, Prof. Dr.
format Article
author Mohd Farhan, Abd Razak
Abd. Razak, Shaari
Pin, Kar Yong
Luqman Chuah, Abdullah, Prof. Dr.
author_sort Mohd Farhan, Abd Razak
title Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
title_short Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
title_full Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
title_fullStr Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
title_full_unstemmed Antioxidant activity and phenolic content of different parts of Orthosiphon stamineus grown under different light intensities
title_sort antioxidant activity and phenolic content of different parts of orthosiphon stamineus grown under different light intensities
publisher Forest Research Institute Malaysia
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/26050
_version_ 1643794822140526592
score 13.214268

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