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Nanobioprobe for the determination of pork adulteration in burger formulations.

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Main Authors: Md. Eaqub, Ali, Shuhaimi, Mustafa, Assoc. Prof Dr., Uda, Hashim, Prof. Dr., Yaakob, Che Man, Prof. Dr, Foo, Kai Loong
Other Authors: uda@unimap.edu.my
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Subjects:
Meats
Beef burger
Nanobioprobe
Pork adulteration
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/26006
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spelling my.unimap-260062014-04-08T08:57:27Z Nanobioprobe for the determination of pork adulteration in burger formulations. Md. Eaqub, Ali Shuhaimi, Mustafa, Assoc. Prof Dr. Uda, Hashim, Prof. Dr. Yaakob, Che Man, Prof. Dr Foo, Kai Loong uda@unimap.edu.my Meats Beef burger Nanobioprobe Pork adulteration Link to publisher's homepage at http://www.hindawi.com/ We report the development of a swine-specific hybrid nanobioprobe through a covalent integration of a fluorophore-labeled 27-nucleotide AluI-fragment of swine cytochrome b gene to a 3nm gold nanoparticle for the determination of pork adulteration in processed meat products. We tested the probe to estimate adulterated pork in ready-to-eat pork-spiked beef burgers. The probe quantitatively detected 1-100% spiked pork in burger formulations with 90% accuracy. A plot of observed fluorescence against the known concentration of AluI-digested pork DNA targets generated a concave curve, demonstrating a power relationship (y=2.956 x 0.509) with a regression coefficient (R 2) of 0.986. No cross-species detection was found in a standard set of pork, beef, chicken, mutton, and chevon burgers. The method is suitable for the determination of very short-length nucleic acid targets which cannot be estimated by conventional and real-time PCR but are essential for the determination of microRNA in biodiagnostics and degraded DNA in forensic testing and food analysis. 2013-06-24T04:23:53Z 2013-06-24T04:23:53Z 2012 Article Journal of Nanomaterials, vol. 2012, 2012, pages 1-7 1687-4110 http://www.hindawi.com/journals/jnm/2012/832387/ http://hdl.handle.net/123456789/26006 en Hindawi Publishing Corporation
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Meats
Beef burger
Nanobioprobe
Pork adulteration
spellingShingle Meats
Beef burger
Nanobioprobe
Pork adulteration
Md. Eaqub, Ali
Shuhaimi, Mustafa, Assoc. Prof Dr.
Uda, Hashim, Prof. Dr.
Yaakob, Che Man, Prof. Dr
Foo, Kai Loong
Nanobioprobe for the determination of pork adulteration in burger formulations.
description Link to publisher's homepage at http://www.hindawi.com/
author2 uda@unimap.edu.my
author_facet uda@unimap.edu.my
Md. Eaqub, Ali
Shuhaimi, Mustafa, Assoc. Prof Dr.
Uda, Hashim, Prof. Dr.
Yaakob, Che Man, Prof. Dr
Foo, Kai Loong
format Article
author Md. Eaqub, Ali
Shuhaimi, Mustafa, Assoc. Prof Dr.
Uda, Hashim, Prof. Dr.
Yaakob, Che Man, Prof. Dr
Foo, Kai Loong
author_sort Md. Eaqub, Ali
title Nanobioprobe for the determination of pork adulteration in burger formulations.
title_short Nanobioprobe for the determination of pork adulteration in burger formulations.
title_full Nanobioprobe for the determination of pork adulteration in burger formulations.
title_fullStr Nanobioprobe for the determination of pork adulteration in burger formulations.
title_full_unstemmed Nanobioprobe for the determination of pork adulteration in burger formulations.
title_sort nanobioprobe for the determination of pork adulteration in burger formulations.
publisher Hindawi Publishing Corporation
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/26006
_version_ 1643794807823269888
score 13.214268

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