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Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation

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Main Authors: Md Eaqub, Ali, Uda, Hashim, Prof. Dr., Shuhaimi, Mustafa, Assoc. Prof. Dr., Yaakob, Che Man, Prof. Datuk Dr., Kh. N. Islam
Other Authors: uda@unimap.edu.my
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Subjects:
Gold nanoparticles (GNPs)
Pork adulteration
Food analysis
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/26005
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spelling my.unimap-260052013-06-21T09:38:05Z Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation Md Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaakob, Che Man, Prof. Datuk Dr. Kh. N. Islam uda@unimap.edu.my Gold nanoparticles (GNPs) Pork adulteration Food analysis Link to publisher's homepage at http://www.hindawi.com/ We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards. Twenty nm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525 nm is red-shifted by 30–50 nm in 3 mM phosphate buffer saline (PBS). Adsorption of single-stranded DNA protects the particles against salt-induced aggregation. Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature. The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation. Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation. This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550–800 nm regimes. This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies. 2013-06-21T09:38:05Z 2013-06-21T09:38:05Z 2012 Article Journal of Nanomaterials, vol. 2012, 2012, pages 1-7 1687-4110 http://www.hindawi.com/journals/jnm/2012/103607/ http://hdl.handle.net/123456789/26005 en Hindawi Publishing Corporation
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Gold nanoparticles (GNPs)
Pork adulteration
Food analysis
spellingShingle Gold nanoparticles (GNPs)
Pork adulteration
Food analysis
Md Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaakob, Che Man, Prof. Datuk Dr.
Kh. N. Islam
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
description Link to publisher's homepage at http://www.hindawi.com/
author2 uda@unimap.edu.my
author_facet uda@unimap.edu.my
Md Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaakob, Che Man, Prof. Datuk Dr.
Kh. N. Islam
format Article
author Md Eaqub, Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa, Assoc. Prof. Dr.
Yaakob, Che Man, Prof. Datuk Dr.
Kh. N. Islam
author_sort Md Eaqub, Ali
title Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
title_short Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
title_full Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
title_fullStr Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
title_full_unstemmed Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
title_sort gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
publisher Hindawi Publishing Corporation
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/26005
_version_ 1643794807375527936
score 13.214268

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