Development of swine-specific DNA markers for biosensor-based halal authentication
Link to publisher's homepage at http://www.funpecrp.com.br/
Saved in:
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Fundacao de Pesquisas Cientificas de Ribeirao Preto (FUNPEC-RO)
2013
|
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my/xmlui/handle/123456789/23666 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.unimap-23666 |
|---|---|
| record_format |
dspace |
| spelling |
my.unimap-236662013-02-19T05:49:17Z Development of swine-specific DNA markers for biosensor-based halal authentication Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Muhammad, Kashif Shuhaimi, Mustafa, Assoc. Prof Dr. Yaakob, Che Man, Prof. Dr. eaqubali@gmail.com uda@unimap.edu.my kashif_bme@yahoo.com shuhaimi@biotech.upm.edu.my ycm@putra.upm.edu.my Amplicon length In silico digestion Intra- and interspecies polymorphisms Mitochondrial genome Protein coding genes Restriction enzyme Link to publisher's homepage at http://www.funpecrp.com.br/ The pig (Sus scrofa) mitochondrial genome was targeted to design short (15-30 nucleotides) DNA markers that would be suitable for biosensor-based hybridization detection of target DNA. Short DNA markers are reported to survive harsh conditions in which longer ones are degraded into smaller fragments. The whole swine mitochondrial-genome was in silico digested with AluI restriction enzyme. Among 66 AluI fragments, five were selected as potential markers because of their convenient lengths, high degree of interspecies polymorphism and intraspecies conservatism. These were confirmed by NCBI blast analysis and ClustalW alignment analysis with 11 different meat-providing animal and fish species. Finally, we integrated a tetramethyl rhodamine-labeled 18-nucleotide AluI fragment into a 3-nm diameter citrate-tannate coated gold nanoparticle to develop a swine-specific hybrid nanobioprobe for the determination of pork adulteration in 2.5-h autoclaved pork-beef binary mixtures. This hybrid probe detected as low as 1% pork in deliberately contaminated autoclaved pork-beef binary mixtures and no cross-species detection was recorded, demonstrating the feasibility of this type of probe for biosensor-based detection of pork adulteration of halal and kosher foods. 2013-02-19T05:49:17Z 2013-02-19T05:49:17Z 2012-06 Article Genetic and Molecular Research, 11 (2), 2012, pages 1762-1772. 1676-5680 http://www.geneticsmr.com//year2012/vol11-2/pdf/gmr1748.pdf http://hdl.handle.net/123456789/23666 en Fundacao de Pesquisas Cientificas de Ribeirao Preto (FUNPEC-RO) |
| institution |
Universiti Malaysia Perlis |
| building |
UniMAP Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaysia Perlis |
| content_source |
UniMAP Library Digital Repository |
| url_provider |
http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Amplicon length In silico digestion Intra- and interspecies polymorphisms Mitochondrial genome Protein coding genes Restriction enzyme |
| spellingShingle |
Amplicon length In silico digestion Intra- and interspecies polymorphisms Mitochondrial genome Protein coding genes Restriction enzyme Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Muhammad, Kashif Shuhaimi, Mustafa, Assoc. Prof Dr. Yaakob, Che Man, Prof. Dr. Development of swine-specific DNA markers for biosensor-based halal authentication |
| description |
Link to publisher's homepage at http://www.funpecrp.com.br/ |
| author2 |
eaqubali@gmail.com |
| author_facet |
eaqubali@gmail.com Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Muhammad, Kashif Shuhaimi, Mustafa, Assoc. Prof Dr. Yaakob, Che Man, Prof. Dr. |
| format |
Article |
| author |
Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Muhammad, Kashif Shuhaimi, Mustafa, Assoc. Prof Dr. Yaakob, Che Man, Prof. Dr. |
| author_sort |
Md. Eaqub, Ali |
| title |
Development of swine-specific DNA markers for biosensor-based halal authentication |
| title_short |
Development of swine-specific DNA markers for biosensor-based halal authentication |
| title_full |
Development of swine-specific DNA markers for biosensor-based halal authentication |
| title_fullStr |
Development of swine-specific DNA markers for biosensor-based halal authentication |
| title_full_unstemmed |
Development of swine-specific DNA markers for biosensor-based halal authentication |
| title_sort |
development of swine-specific dna markers for biosensor-based halal authentication |
| publisher |
Fundacao de Pesquisas Cientificas de Ribeirao Preto (FUNPEC-RO) |
| publishDate |
2013 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/23666 |
| _version_ |
1643794072409735168 |
| score |
13.214268 |