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NanoDNA chip for the detection of porcine derivatives in halal products

International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia.

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Bibliographic Details
Main Authors: Md. Eaqub, Ali, Uda, Hashim, Prof. Dr., Md. Fazlul, Bari, Dr.
Other Authors: eaqubali@gmail.com
Format: Working Paper
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2012
Subjects:
NanoDNA chip
Label free electrical detection
Nanogap electrodes
Conserved sequences
Spacer process lithography
AluI sites
AluI digestion
Polymorphism
Blast
Alignment analysis
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/21499
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spelling my.unimap-214992012-10-21T08:54:07Z NanoDNA chip for the detection of porcine derivatives in halal products Md. Eaqub, Ali Uda, Hashim, Prof. Dr. Md. Fazlul, Bari, Dr. eaqubali@gmail.com NanoDNA chip Label free electrical detection Nanogap electrodes Conserved sequences Spacer process lithography AluI sites AluI digestion Polymorphism Blast Alignment analysis International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia. Pig (Sus scrofa) mitochondrial genome was analyzed for AluI sites by NEB cutter version 2 and REBASE softwares. Out of 66 AluI fragments, five were selected as probes for the construction of porcine mitochondrial DNA chip because of their convenient sizes, highly conserved unique specificity for porcine mitochondrial genome and polymorphism with other species. These were confirmed by NCBI blast analysis and ClustalW alignment analysis. These AluI cut fragments are proposed to be immobilized on nanogap electrodes to be fabricated by spacer process lithography to construct NanoDNA chip. Application of AluI digested denatured ssDNA on the chip will immediately hybridized with the immobilized probes to form dsDNA to bring electronic and electrical changes in the dielectric properties of the molecule. The electronic and electrical signals will be read by a dielectric analyzer to detect the presence of porcine DNA with any additional labeling steps. 2012-10-21T08:54:07Z 2012-10-21T08:54:07Z 2010-10-16 Working Paper 978-967-5760-03-7 http://hdl.handle.net/123456789/21499 en Proceedings of the International Postgraduate Conference on Engineering (IPCE 2010) Universiti Malaysia Perlis (UniMAP) Centre for Graduate Studies
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic NanoDNA chip
Label free electrical detection
Nanogap electrodes
Conserved sequences
Spacer process lithography
AluI sites
AluI digestion
Polymorphism
Blast
Alignment analysis
spellingShingle NanoDNA chip
Label free electrical detection
Nanogap electrodes
Conserved sequences
Spacer process lithography
AluI sites
AluI digestion
Polymorphism
Blast
Alignment analysis
Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Md. Fazlul, Bari, Dr.
NanoDNA chip for the detection of porcine derivatives in halal products
description International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia.
author2 eaqubali@gmail.com
author_facet eaqubali@gmail.com
Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Md. Fazlul, Bari, Dr.
format Working Paper
author Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Md. Fazlul, Bari, Dr.
author_sort Md. Eaqub, Ali
title NanoDNA chip for the detection of porcine derivatives in halal products
title_short NanoDNA chip for the detection of porcine derivatives in halal products
title_full NanoDNA chip for the detection of porcine derivatives in halal products
title_fullStr NanoDNA chip for the detection of porcine derivatives in halal products
title_full_unstemmed NanoDNA chip for the detection of porcine derivatives in halal products
title_sort nanodna chip for the detection of porcine derivatives in halal products
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/21499
_version_ 1643793377485914112
score 13.222552

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