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Isolation and morphological characterisation of bovine adipose tissue derived stem cells

International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia.

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Main Authors: Fhataheya, Buang, Mohamed Zulkali, Mohamed Daud, Prof. Madya Dr., Ku Syahidah, Ku Ismail, Ruszymah, Hj Idrus, Mukhzeer, Mohamad Shahimin, Dr.
Other Authors: fhataheya_buang@yahoo.com
Format: Working Paper
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2012
Subjects:
Bovine adipose tissues
Tissue engineering
Stem cells
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/21238
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spelling my.unimap-212382012-10-08T03:48:00Z Isolation and morphological characterisation of bovine adipose tissue derived stem cells Fhataheya, Buang Mohamed Zulkali, Mohamed Daud, Prof. Madya Dr. Ku Syahidah, Ku Ismail Ruszymah, Hj Idrus Mukhzeer, Mohamad Shahimin, Dr. fhataheya_buang@yahoo.com Bovine adipose tissues Tissue engineering Stem cells International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia. Bovine adipose tissue derived stem cells have a tremendous potential in regenerative medicine for tissue engineering, especially in Somatic Nuclear Transfer (SNT) for making transgenic animal and animal breeding. The optimisation of bovine tissue stem cells isolation will maximise the quantity of stem cells. This directly reduces the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Abdominal subcutaneous and inguinal adipose tissue are collected and digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1.5 hours and 2 hours under 370C in 5% CO2 incubator. The cultures are morphologically characterised. Bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 hours under 37 0C in CO2 incubator. 2012-10-08T03:48:00Z 2012-10-08T03:48:00Z 2010-10-16 Working Paper 978-967-5760-03-7 http://hdl.handle.net/123456789/21238 en Proceedings of the International Postgraduate Conference on Engineering (IPCE 2010) Universiti Malaysia Perlis (UniMAP) Centre for Graduate Studies
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bovine adipose tissues
Tissue engineering
Stem cells
spellingShingle Bovine adipose tissues
Tissue engineering
Stem cells
Fhataheya, Buang
Mohamed Zulkali, Mohamed Daud, Prof. Madya Dr.
Ku Syahidah, Ku Ismail
Ruszymah, Hj Idrus
Mukhzeer, Mohamad Shahimin, Dr.
Isolation and morphological characterisation of bovine adipose tissue derived stem cells
description International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia.
author2 fhataheya_buang@yahoo.com
author_facet fhataheya_buang@yahoo.com
Fhataheya, Buang
Mohamed Zulkali, Mohamed Daud, Prof. Madya Dr.
Ku Syahidah, Ku Ismail
Ruszymah, Hj Idrus
Mukhzeer, Mohamad Shahimin, Dr.
format Working Paper
author Fhataheya, Buang
Mohamed Zulkali, Mohamed Daud, Prof. Madya Dr.
Ku Syahidah, Ku Ismail
Ruszymah, Hj Idrus
Mukhzeer, Mohamad Shahimin, Dr.
author_sort Fhataheya, Buang
title Isolation and morphological characterisation of bovine adipose tissue derived stem cells
title_short Isolation and morphological characterisation of bovine adipose tissue derived stem cells
title_full Isolation and morphological characterisation of bovine adipose tissue derived stem cells
title_fullStr Isolation and morphological characterisation of bovine adipose tissue derived stem cells
title_full_unstemmed Isolation and morphological characterisation of bovine adipose tissue derived stem cells
title_sort isolation and morphological characterisation of bovine adipose tissue derived stem cells
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/21238
_version_ 1643793314771632128
score 13.222552

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