Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis

International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Kampus Pauh Putra, Arau, Perlis, Malaysia.

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Main Authors: Azlinda, Abdul Ghani, Zarina, Zakaria, Dr., Ku Syahidah, Ku Ismail, Nor Alina, Nazri
Other Authors: azlinda1903@yahoo.com
Format: Working Paper
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2012
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Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/21210
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spelling my.unimap-212102012-10-05T02:11:41Z Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis Azlinda, Abdul Ghani Zarina, Zakaria, Dr. Ku Syahidah, Ku Ismail Nor Alina, Nazri azlinda1903@yahoo.com Flavonones compounds Callus culture Antioxidants Citrus grandis International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Kampus Pauh Putra, Arau, Perlis, Malaysia. This paper discus on the production of flavonones, one of the flavonoids compounds that correlated with antioxidants activities in callus culture of pomelo. This is an alternative strategy of producing natural antioxidants without sacrificing part of plants. Flavonones was detected as naringin and hesperidin using High Performance Liquid Chromatography (HPLC). There are three types of media were used to investigate their capability to produce the highest callus biomass. It was found that growth of callus was preferable on medium supplied with 1925 mg/l of nitrate and 60 g/l of sucrose. For both combinations, the result showed that it can give the best hesperidin accumulation (22.861 and 69.923 mg/l). The maximum productivity of hesperidin can be accumulated by seven days of incubation period. Naringin however was maximize with supplementation of 1925 mg/l nitrate (18.676 mg/l) and 90 g/l (33.876 mg/l) sucrose within the same duration time of incubation period. 2012-10-05T02:10:44Z 2012-10-05T02:10:44Z 2010-10-16 Working Paper 978-967-5760-03-7 http://hdl.handle.net/123456789/21210 en Proceedings of the International Postgraduate Conference on Engineering (IPCE 2010) Universiti Malaysia Perlis (UniMAP) Centre for Graduate Studies
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Flavonones compounds
Callus culture
Antioxidants
Citrus grandis
spellingShingle Flavonones compounds
Callus culture
Antioxidants
Citrus grandis
Azlinda, Abdul Ghani
Zarina, Zakaria, Dr.
Ku Syahidah, Ku Ismail
Nor Alina, Nazri
Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
description International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Kampus Pauh Putra, Arau, Perlis, Malaysia.
author2 azlinda1903@yahoo.com
author_facet azlinda1903@yahoo.com
Azlinda, Abdul Ghani
Zarina, Zakaria, Dr.
Ku Syahidah, Ku Ismail
Nor Alina, Nazri
format Working Paper
author Azlinda, Abdul Ghani
Zarina, Zakaria, Dr.
Ku Syahidah, Ku Ismail
Nor Alina, Nazri
author_sort Azlinda, Abdul Ghani
title Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
title_short Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
title_full Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
title_fullStr Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
title_full_unstemmed Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
title_sort effect of nutrient modification on growth and flavonone production from callus culture of citrus grandis
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/21210
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score 13.214268