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Isolation methods of human and bovine adipose tissue derived stem cells

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Main Authors: Fhataheya, Buang, Mukhzeer, Mohamad Shahimin, Dr.
Other Authors: mukhzeer@unimap.edu.my
Format: Working Paper
Language:English
Published: Institute of Electrical and Electronics Engineers (IEEE) 2012
Subjects:
Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/18753
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spelling my.unimap-187532012-04-10T12:36:09Z Isolation methods of human and bovine adipose tissue derived stem cells Fhataheya, Buang Mukhzeer, Mohamad Shahimin, Dr. mukhzeer@unimap.edu.my fhataheya_buang@yahoo.com Abdominal subcutaneous adipose tissue Bovine Human Stem cell isolation Link to publisher's homepage at http://www.ieee.org/ Mammalian adipose tissue derived stem cells have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1 hour and 2 hours under 37°C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1 hours under 37C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 hours under 37°C in CO2 incubator. 2012-04-10T12:36:09Z 2012-04-10T12:36:09Z 2010-11-30 Working Paper p. 176-179 978-1-4244-7600-8 http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5742223 http://hdl.handle.net/123456789/18753 en Proceedings of 2010 IEEE EMBS Conference on Biomedical Engineering and Sciences (IECBES) 2010 Institute of Electrical and Electronics Engineers (IEEE)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
spellingShingle Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
Fhataheya, Buang
Mukhzeer, Mohamad Shahimin, Dr.
Isolation methods of human and bovine adipose tissue derived stem cells
description Link to publisher's homepage at http://www.ieee.org/
author2 mukhzeer@unimap.edu.my
author_facet mukhzeer@unimap.edu.my
Fhataheya, Buang
Mukhzeer, Mohamad Shahimin, Dr.
format Working Paper
author Fhataheya, Buang
Mukhzeer, Mohamad Shahimin, Dr.
author_sort Fhataheya, Buang
title Isolation methods of human and bovine adipose tissue derived stem cells
title_short Isolation methods of human and bovine adipose tissue derived stem cells
title_full Isolation methods of human and bovine adipose tissue derived stem cells
title_fullStr Isolation methods of human and bovine adipose tissue derived stem cells
title_full_unstemmed Isolation methods of human and bovine adipose tissue derived stem cells
title_sort isolation methods of human and bovine adipose tissue derived stem cells
publisher Institute of Electrical and Electronics Engineers (IEEE)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/18753
_version_ 1643792518569000960
score 13.214268

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