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Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste

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Main Author: Nurul Afidah, Umoruddin
Other Authors: Syazni Zainul Kamal
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Cellulases
Aspergillus niger
Hydrolytic enzymes
Cassava waste
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/17073
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spelling my.unimap-170732011-12-08T07:49:17Z Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste Nurul Afidah, Umoruddin Syazni Zainul Kamal Cellulases Aspergillus niger Hydrolytic enzymes Cassava waste Access is limited to UniMAP community. Cellulases are a group of hydrolytic enzymes and are capable of degrading lignocellulosic materials contain carbohydrate polymers such as cellulose, hemicelulose and starch. Cellulases have wide range of applications. In this study, cellulase production was carried out in solid-state fermentation using cassava waste as a substrate by using Aspergillus niger. Different fermentation conditions were examined to assess their effect in optimizing enzyme production. The effects of various process variables were studied for optimum production of cellulase enzyme using cassava waste including moisture content (%), fermentation time (day) and inoculums size (ml). Fermentation of A.niger with cassava waste substrate will produce cellulases. Cellulases enzyme will degrade cellulose to the smaller glucose units. The production of cellulase was determined based on glucose concentration. Full factorial composite experimental design and central composite design (CCD) were used in the design of experiments and analysis of results. The result indicated that the optimal condition for fermentation of 5 g cassava waste substrate by A.niger was obtained at 100 % of moisture content, 7.95 days of fermentation and 3.78 ml of inoculums size. The coefficient of determination obtained was 0.9125 shows the model adequacy was very satisfactory. 2011-12-08T07:49:16Z 2011-12-08T07:49:16Z 2011-04 Learning Object http://hdl.handle.net/123456789/17073 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Cellulases
Aspergillus niger
Hydrolytic enzymes
Cassava waste
spellingShingle Cellulases
Aspergillus niger
Hydrolytic enzymes
Cassava waste
Nurul Afidah, Umoruddin
Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
description Access is limited to UniMAP community.
author2 Syazni Zainul Kamal
author_facet Syazni Zainul Kamal
Nurul Afidah, Umoruddin
format Learning Object
author Nurul Afidah, Umoruddin
author_sort Nurul Afidah, Umoruddin
title Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
title_short Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
title_full Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
title_fullStr Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
title_full_unstemmed Physical parameter optimization of cellulase production by Aspergillus niger on solid-state fermentation of cassava waste
title_sort physical parameter optimization of cellulase production by aspergillus niger on solid-state fermentation of cassava waste
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/17073
_version_ 1643791032803917824
score 13.214268

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