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Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa

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Bibliographic Details
Main Author: Tham, Chee Fai
Other Authors: Rahimah Othman
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Shaker flask
2 L bioreactor
Fermentation
kLa
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/16767
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spelling my.unimap-167672011-12-05T05:44:42Z Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa Tham, Chee Fai Rahimah Othman Shaker flask 2 L bioreactor Fermentation kLa Access is limited to UniMAP community. This study is carried out to investigate gas-liquid mass transfer process in a shaker flask and a 2 L bioreactor. Deionized water was used as based medium whereas shaker flask was scaled up to a 2 L bioreactor scale by using method of constant oxygen mass transfer coefficient, kLa. kLa values were derived by fitting the mass transfer equation to the data of dissolved oxygen percentage (DO) versus time. Screening of Design of Experiment (DOE) was performed and analysis of variance (ANOVA) was run in order to determine the significance of factors that affecting the kLa value. Based on ANOVA, stirring speed and aeration rate were significant factors for both shaker flask and 2 L bioreactor scales but temperature was not. A regression equation was generated for both shaker flask and 2 L bioreactor scales. For shaker flask, 666 rpm of stirring speed with 1 LPM aeration rate was required to obtain 0.5 min-1 of kLa value. Meanwhile, for 2 L bioreactor, 1 LPM of aeration rate without stirring was required to obtain same value of kLa. At the end of this study, shaker flask was successfully scaled up to 2 L bioreactor scale with constant kLa and the significance of factors was studied. 2011-12-05T05:44:42Z 2011-12-05T05:44:42Z 2011-05 Learning Object http://hdl.handle.net/123456789/16767 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Shaker flask
2 L bioreactor
Fermentation
kLa
spellingShingle Shaker flask
2 L bioreactor
Fermentation
kLa
Tham, Chee Fai
Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
description Access is limited to UniMAP community.
author2 Rahimah Othman
author_facet Rahimah Othman
Tham, Chee Fai
format Learning Object
author Tham, Chee Fai
author_sort Tham, Chee Fai
title Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
title_short Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
title_full Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
title_fullStr Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
title_full_unstemmed Scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kLa
title_sort scale-up from shaker flask to stirred-tank fermenter based on mass transfer coefficient of oxygen, kla
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/16767
_version_ 1643791064107057152
score 13.214268

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