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Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium

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Main Author: Suraya, Mohd Tajuddin
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Lignin peroxidase (ligninase)
Rice straw
Fermentation
Biotechnology
Phanerochaete chrysosporium
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/16519
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spelling my.unimap-165192011-12-01T03:43:21Z Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium Suraya, Mohd Tajuddin Mohamad Fahrurrazi Tompang Lignin peroxidase (ligninase) Rice straw Fermentation Biotechnology Phanerochaete chrysosporium Access is limited to UniMAP community. A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus, Phanerochaete chrysosporium ATCC 24725 using rice straw as the major substrate. The optimization media was done using Central Composite Design (CCD) under Response Surface Model (RSM) from Design Expert ™ software, which involved glucose, peptone concentration and inoculum size as the parameters. Nineteen experimental runs were carried out for five days of incubation under condition of 32 oC and 70 % of moisture content. Then, second-order of quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of the parameters. Analysis of variance (ANOVA) showed a high coefficient of determination R2 value of 0.9967 thus indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum media compositions were determined to be 1.0 % (w/v) glucose, 0.3% (w/v) peptone and 10 % (w/v) inoculum size with a maximum activity of 956.96 U/ml ligninase. The validation experiment showed the maximum activity of lignin peroxidase was 968.2 U/ml after 5 days of fermentation which is 1.27 % higher than the predicted value. 2011-12-01T03:43:21Z 2011-12-01T03:43:21Z 2011-05 Learning Object http://hdl.handle.net/123456789/16519 en Universiti Malaysia Perlis School Of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lignin peroxidase (ligninase)
Rice straw
Fermentation
Biotechnology
Phanerochaete chrysosporium
spellingShingle Lignin peroxidase (ligninase)
Rice straw
Fermentation
Biotechnology
Phanerochaete chrysosporium
Suraya, Mohd Tajuddin
Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Suraya, Mohd Tajuddin
format Learning Object
author Suraya, Mohd Tajuddin
author_sort Suraya, Mohd Tajuddin
title Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
title_short Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
title_full Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
title_fullStr Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
title_full_unstemmed Media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
title_sort media optimization for ligninase production from rice straw by solid state fermentation using phanarochate chrysosporium
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/16519
_version_ 1643791062024585216
score 13.160551

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