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Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products

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Main Authors: M. Eaqub, Ali, Uda, Hashim, Prof. Dr., S., Mustafa, Yaakob, Che Man
Format: Article
Language:English
Published: Springerlink 2011
Subjects:
Chip-based capillary electrophoresis
Halal authentication
Ternary mixtures
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/14182
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spelling my.unimap-141822013-11-20T03:57:31Z Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products M. Eaqub, Ali Uda, Hashim, Prof. Dr. S., Mustafa Yaakob, Che Man Chip-based capillary electrophoresis Halal authentication Ternary mixtures Link to publisher's homepage at http://www.springerlink.com Verification of pork adulteration in commercial meat products is increasingly important for the authentication of Halal labels in processed foods. Here, we documented a PCR-restriction fragment length polymorphism (RFLP) assay with high precision and reproducibility for the tracing of porcine DNA in commercial meat products. The assay combined the species-specific primers to selectively amplify a short fragment (109 bp) of porcine cytochrome b gene from a heterogeneous background of genomic DNAs followed by RFLP analysis to authenticate real amplicon. The analysis of PCR products and restriction digests was automated in a chip-based capillary electrophoresis incorporated in Agilent 2100 bioanalyzer. The swine specificity of the assay was checked with 11 different meat-providing animal and fish species. Model experiments, mimicking the processed foods, were performed in binary and ternary mixtures after mechanical grinding and prolonged autoclaving. Finally, four types of the most popular finished meat products (meatball, streaky beacon, frankfurter, and burger) which are prevalent in the Malaysian food market were analyzed in order to verify the assay performance. The assay was sensitive enough to detect 0.0001 ng of swine DNA in pure formats and 0.01% (w/w) spiked pork in extensively processed ternary mixture of pork, beef, and wheat flour. 2011-10-13T00:56:01Z 2011-10-13T00:56:01Z 2011 Article Food Analytical Methods, 2011, pages 1-11 1936-9751 http://www.springerlink.com/content/t546jq4j425g03n5/ http://hdl.handle.net/123456789/14182 en Springerlink
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Chip-based capillary electrophoresis
Halal authentication
Ternary mixtures
spellingShingle Chip-based capillary electrophoresis
Halal authentication
Ternary mixtures
M. Eaqub, Ali
Uda, Hashim, Prof. Dr.
S., Mustafa
Yaakob, Che Man
Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
description Link to publisher's homepage at http://www.springerlink.com
format Article
author M. Eaqub, Ali
Uda, Hashim, Prof. Dr.
S., Mustafa
Yaakob, Che Man
author_facet M. Eaqub, Ali
Uda, Hashim, Prof. Dr.
S., Mustafa
Yaakob, Che Man
author_sort M. Eaqub, Ali
title Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
title_short Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
title_full Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
title_fullStr Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
title_full_unstemmed Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products
title_sort swine-specific pcr-rflp assay targeting mitochondrial cytochrome b gene for semiquantitative detection of pork in commercial meat products
publisher Springerlink
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/14182
_version_ 1643791199032573952
score 13.214268

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