Optimisation of hydrolysis conditions for ethanol production from sorghum starch
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The Institution of Engineers, Malaysia
2011
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my.unimap-137032011-09-10T08:06:34Z Optimisation of hydrolysis conditions for ethanol production from sorghum starch Najiah, Nadir Maizirwan, Mel, Dr. Mohamed Ismail, Abdul Karim, Prof. Dr. Rosli, Mohd Yunus, Prof. Dr. najdir@yahoo.com α-amylase Glucoamylase Liquefaction Optimisation Saccharification Sorghum starch Link to publisher's homepage at http://www.myiem.org.my/ The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance (ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. 2011-09-10T08:06:34Z 2011-09-10T08:06:34Z 2010-09 Article The Journal of the Institution of Engineers, Malaysia, vol. 71(3), 2010, pages 26-34 0126-513X http://www.myiem.org.my/content/iem_journal_2010-181.aspx http://hdl.handle.net/123456789/13703 en The Institution of Engineers, Malaysia |
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English |
| topic |
α-amylase Glucoamylase Liquefaction Optimisation Saccharification Sorghum starch |
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α-amylase Glucoamylase Liquefaction Optimisation Saccharification Sorghum starch Najiah, Nadir Maizirwan, Mel, Dr. Mohamed Ismail, Abdul Karim, Prof. Dr. Rosli, Mohd Yunus, Prof. Dr. Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
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Link to publisher's homepage at http://www.myiem.org.my/ |
| author2 |
najdir@yahoo.com |
| author_facet |
najdir@yahoo.com Najiah, Nadir Maizirwan, Mel, Dr. Mohamed Ismail, Abdul Karim, Prof. Dr. Rosli, Mohd Yunus, Prof. Dr. |
| format |
Article |
| author |
Najiah, Nadir Maizirwan, Mel, Dr. Mohamed Ismail, Abdul Karim, Prof. Dr. Rosli, Mohd Yunus, Prof. Dr. |
| author_sort |
Najiah, Nadir |
| title |
Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_short |
Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_full |
Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_fullStr |
Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_full_unstemmed |
Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_sort |
optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| publisher |
The Institution of Engineers, Malaysia |
| publishDate |
2011 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/13703 |
| _version_ |
1643791001540624384 |
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13.222552 |