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Optimisation of hydrolysis conditions for ethanol production from sorghum starch

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Main Authors: Najiah, Nadir, Maizirwan, Mel, Dr., Mohamed Ismail, Abdul Karim, Prof. Dr., Rosli, Mohd Yunus, Prof. Dr.
Other Authors: najdir@yahoo.com
Format: Article
Language:English
Published: The Institution of Engineers, Malaysia 2011
Subjects:
α-amylase
Glucoamylase
Liquefaction
Optimisation
Saccharification
Sorghum starch
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13703
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spelling my.unimap-137032011-09-10T08:06:34Z Optimisation of hydrolysis conditions for ethanol production from sorghum starch Najiah, Nadir Maizirwan, Mel, Dr. Mohamed Ismail, Abdul Karim, Prof. Dr. Rosli, Mohd Yunus, Prof. Dr. najdir@yahoo.com α-amylase Glucoamylase Liquefaction Optimisation Saccharification Sorghum starch Link to publisher's homepage at http://www.myiem.org.my/ The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance (ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. 2011-09-10T08:06:34Z 2011-09-10T08:06:34Z 2010-09 Article The Journal of the Institution of Engineers, Malaysia, vol. 71(3), 2010, pages 26-34 0126-513X http://www.myiem.org.my/content/iem_journal_2010-181.aspx http://hdl.handle.net/123456789/13703 en The Institution of Engineers, Malaysia
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic α-amylase
Glucoamylase
Liquefaction
Optimisation
Saccharification
Sorghum starch
spellingShingle α-amylase
Glucoamylase
Liquefaction
Optimisation
Saccharification
Sorghum starch
Najiah, Nadir
Maizirwan, Mel, Dr.
Mohamed Ismail, Abdul Karim, Prof. Dr.
Rosli, Mohd Yunus, Prof. Dr.
Optimisation of hydrolysis conditions for ethanol production from sorghum starch
description Link to publisher's homepage at http://www.myiem.org.my/
author2 najdir@yahoo.com
author_facet najdir@yahoo.com
Najiah, Nadir
Maizirwan, Mel, Dr.
Mohamed Ismail, Abdul Karim, Prof. Dr.
Rosli, Mohd Yunus, Prof. Dr.
format Article
author Najiah, Nadir
Maizirwan, Mel, Dr.
Mohamed Ismail, Abdul Karim, Prof. Dr.
Rosli, Mohd Yunus, Prof. Dr.
author_sort Najiah, Nadir
title Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_short Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_fullStr Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full_unstemmed Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_sort optimisation of hydrolysis conditions for ethanol production from sorghum starch
publisher The Institution of Engineers, Malaysia
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13703
_version_ 1643791001540624384
score 13.214268

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