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The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass

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Main Authors: Abu Saleh, Ahmed, Dr., Sinin, Hamdan, Dr., Makino, Keisuke, Prof.
Other Authors: aasaleh@feng.unimas.my
Format: Article
Language:English
Published: The Institution of Engineers, Malaysia 2011
Subjects:
Biofuel
Ethanol production
Lignocellulosic biomass
Xylose-fermentation
Yeast
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13655
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spelling my.unimap-136552011-09-04T05:06:20Z The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass Abu Saleh, Ahmed, Dr. Sinin, Hamdan, Dr. Makino, Keisuke, Prof. aasaleh@feng.unimas.my Biofuel Ethanol production Lignocellulosic biomass Xylose-fermentation Yeast Link to publisher's homepage at http://www.myiem.org.my/ In this study, bioengineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Saccharomyces cerevisiae transformed with the native genes encoding xylose reductase (XR), xylitol dehydrogenase (XDH) from Pichia stipitis and the endogenous gene xylulokinage (XK) ferments xylose to ethanol but has not yet been applied to the industrial bio-process due to the unfavorable excretion of xylitol. Intercellular redox imbalance caused by the different coenzyme specificity of XR (using NADPH/NADH with preference for NADPH) and XDH (exclusively using NAD+) has been thought to be one of the main factors of xylitol excretion. The introduction of NADP+-dependent XDH generated in this study prevented the xylitol excretion probably because of maintaining the intercellular redox balance. Recombinant yeasts were constructed with the genes encoding a wild type XR and the protein engineered XDH (with NADP) of P. stipitis. These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes performed the similar enzyme properties in S. cerevisiae cells, compared those in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared to the reference strain expressing the wild–type XDH. Measurement of intracellular coenzyme concentrations suggested that maintenance of the NADPH/NADP and NADH/NAD ratios are important for efficient ethanol fermentation from biomass sugar xylose by a recombinant S. cerevisiae. 2011-09-04T05:06:20Z 2011-09-04T05:06:20Z 2008-06 Article The Journal of the Institution of Engineers, Malaysia, vol. 69(2), 2008, pages 47-53 0126-513X http://www.myiem.org.my/content/iem_journal_2008-179.aspx http://hdl.handle.net/123456789/13655 en The Institution of Engineers, Malaysia
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biofuel
Ethanol production
Lignocellulosic biomass
Xylose-fermentation
Yeast
spellingShingle Biofuel
Ethanol production
Lignocellulosic biomass
Xylose-fermentation
Yeast
Abu Saleh, Ahmed, Dr.
Sinin, Hamdan, Dr.
Makino, Keisuke, Prof.
The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
description Link to publisher's homepage at http://www.myiem.org.my/
author2 aasaleh@feng.unimas.my
author_facet aasaleh@feng.unimas.my
Abu Saleh, Ahmed, Dr.
Sinin, Hamdan, Dr.
Makino, Keisuke, Prof.
format Article
author Abu Saleh, Ahmed, Dr.
Sinin, Hamdan, Dr.
Makino, Keisuke, Prof.
author_sort Abu Saleh, Ahmed, Dr.
title The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
title_short The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
title_full The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
title_fullStr The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
title_full_unstemmed The development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
title_sort development of a highly efficient fermentation process for biofuel production from lignocellulosic biomass
publisher The Institution of Engineers, Malaysia
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13655
_version_ 1643790948297080832
score 13.214268

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