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Evaluation of bioactive component in Wasabi and other cruciferae

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Bibliographic Details
Main Author: Mohd Zakwan, Nawi
Other Authors: Prof. Madya Dr. Zainab Hamzah (Advisor)
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Wasabia japonica
Wasabi
Cruciferous vegetables
Antioxidant
Medicinal plants
Antibacterial
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13379
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spelling my.unimap-133792011-08-04T05:41:18Z Evaluation of bioactive component in Wasabi and other cruciferae Mohd Zakwan, Nawi Prof. Madya Dr. Zainab Hamzah (Advisor) Wasabia japonica Wasabi Cruciferous vegetables Antioxidant Medicinal plants Antibacterial Access is limited to UniMAP community. Wasabia japonica or wasabi is very popular in Japan while cauliflower (Brassica oleracea var. italica), broccoli (B. oleraceavar. botrytis), White radish (Raphanus sativus) is a very popular plant among Malaysians. This project was carried out to determine the bioactive component in these samples after extraction with two different solvents which are water and ethanol. It is also required to compare which method will give the best yield of antioxidant after the extraction. Water extraction method was better compared to ethanol extraction. The compound and type of bond in the extraction samples was also determined by using the Fourier-Transform Infrared (FTIR). Listerine sample was used as a control for antiseptic and antibacterial agents. Moreover, extract of these edible plants also were determined for their antibacterial activity against grampositive and gram-negative bacteria. It is also tested on fungi and yeast as revealed by Kirby-Bauer method. From FTIR study the OH groups present denotes the presence of alcohol and phenolic compounds in the Listerine as well as sample especially in wasabi and radish showing the presence of antiseptic and antibacterial in the sample studied. The Kirby-Bauer method measured antibacterial activity against gram-positive and gram-negative bacteria. Isothiocyanate analysis was not able to determine using the HPLC method due to unavailability of the standards. Other active ingredient such as Rosmarinic acid, 3-Hydroxy 5,6,7,9 tetramethylflavone (TMF), Sinensitin and Eupatorin were determined. 2011-08-04T05:41:18Z 2011-08-04T05:41:18Z 2011-05 Learning Object http://hdl.handle.net/123456789/13379 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Wasabia japonica
Wasabi
Cruciferous vegetables
Antioxidant
Medicinal plants
Antibacterial
spellingShingle Wasabia japonica
Wasabi
Cruciferous vegetables
Antioxidant
Medicinal plants
Antibacterial
Mohd Zakwan, Nawi
Evaluation of bioactive component in Wasabi and other cruciferae
description Access is limited to UniMAP community.
author2 Prof. Madya Dr. Zainab Hamzah (Advisor)
author_facet Prof. Madya Dr. Zainab Hamzah (Advisor)
Mohd Zakwan, Nawi
format Learning Object
author Mohd Zakwan, Nawi
author_sort Mohd Zakwan, Nawi
title Evaluation of bioactive component in Wasabi and other cruciferae
title_short Evaluation of bioactive component in Wasabi and other cruciferae
title_full Evaluation of bioactive component in Wasabi and other cruciferae
title_fullStr Evaluation of bioactive component in Wasabi and other cruciferae
title_full_unstemmed Evaluation of bioactive component in Wasabi and other cruciferae
title_sort evaluation of bioactive component in wasabi and other cruciferae
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13379
_version_ 1643790822367297536
score 13.214268

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