Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas

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Main Author: Mohamad Syafiq, Sa’odi
Other Authors: Dr. AKM Shafiqul Islam (Advisor)
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13364
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spelling my.unimap-133642011-08-02T02:17:09Z Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas Mohamad Syafiq, Sa’odi Dr. AKM Shafiqul Islam (Advisor) Enzymatic biosensor Xanthine Oxidase (XO) Antioxidant Herbal tea Medicinal plants Biosensor Access is limited to UniMAP community. An enzymatic biosensor has been developed using Xanthine Oxidase (XO enzyme as sensing material for the determination of antioxidant properties of selected herbal teas. The enzyme was immobilized in a Kappa-Carrageenan membrane on a carbon paste electrode. The variables concerning the biosensor preparation such as types of tea, amount of Kappa- Carrageenan membrane, concentration of tea samples, addition of Xanthine solution and tea samples, and also addition of supporting electrolytes were studied. The antioxidant properties of the herbal teas were demonstrated by comparing percentage of inhibition of selected tea samples using the standard 1, 1-diphenyl-2-picrylhydrazyl DPPH free radical scavenging potential and with the cyclic voltammogtram using the Dropsens biosensor. Results show that cyclic voltammetry analysis using the Xanthine Oxidase biosensor produced significant result in comparison with the free radical scavenging activity using DPPH. Even though the results using biosensor is less accurate compared to standard method, but it was fairly acceptable. 2011-08-02T02:17:09Z 2011-08-02T02:17:09Z 2011-05 Learning Object http://hdl.handle.net/123456789/13364 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Enzymatic biosensor
Xanthine Oxidase (XO)
Antioxidant
Herbal tea
Medicinal plants
Biosensor
spellingShingle Enzymatic biosensor
Xanthine Oxidase (XO)
Antioxidant
Herbal tea
Medicinal plants
Biosensor
Mohamad Syafiq, Sa’odi
Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
description Access is limited to UniMAP community.
author2 Dr. AKM Shafiqul Islam (Advisor)
author_facet Dr. AKM Shafiqul Islam (Advisor)
Mohamad Syafiq, Sa’odi
format Learning Object
author Mohamad Syafiq, Sa’odi
author_sort Mohamad Syafiq, Sa’odi
title Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
title_short Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
title_full Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
title_fullStr Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
title_full_unstemmed Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas
title_sort development of disposable xanthine oxidase biosensor for the determination of antioxidant properties of herbal teas
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13364
_version_ 1643790819427090432
score 13.214268