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Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant

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Bibliographic Details
Main Author: Abdul Malek, Abdullah
Other Authors: Dr. AKM Shafiqul Islam (Advisor)
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Antioxidant
Biosensor
Tea plant
Superoxide dismutase (SOD) enzyme
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13352
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spelling my.unimap-133522011-07-30T07:56:32Z Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant Abdul Malek, Abdullah Dr. AKM Shafiqul Islam (Advisor) Antioxidant Biosensor Tea plant Superoxide dismutase (SOD) enzyme Access is limited to UniMAP community. A disposable enzymatic biosensor has been developed using superoxide dismutase (SOD) enzyme as its sensing material for the determination of antioxidant capacity of tea samples. The SOD was physically immobilized in a gel called Kappa-carrageenan membrane which suitable for enzyme entrapment. The usefulness of the biosensor for the food analysis was demonstrated by determining the percentage of inhibition of tea samples by using cyclic voltametric method with DropSens Electrochemical cells instrument and compared to the standard method, UV-VIS Spectrophotometry. Design of disposable electrode was suggested to be fabricated by using polyvinyl chloride (PVC). The antioxidant capacity of tea sample can be determined by disposable superoxide dismutase biosensor by the comparison with DPPH reaction by spectrophotometry. Qualitatively, the antioxidants in tea samples show the good results by oxidation and reduction curve of cyclic voltamogram. Quantitatively, the inhibition percentage of antioxidants shows by standard spectrophotometry method were 28.15 %, 34.99 %, 70.03 %, 85.97 % and 90.01 %, when the concentration of tea sample increases. While for biosensor were 10.68 %, 21.72 %, 30.92 %, 41.46 % and 49.45 %. 2011-07-30T07:56:32Z 2011-07-30T07:56:32Z 2011-05 Learning Object http://hdl.handle.net/123456789/13352 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antioxidant
Biosensor
Tea plant
Superoxide dismutase (SOD) enzyme
spellingShingle Antioxidant
Biosensor
Tea plant
Superoxide dismutase (SOD) enzyme
Abdul Malek, Abdullah
Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
description Access is limited to UniMAP community.
author2 Dr. AKM Shafiqul Islam (Advisor)
author_facet Dr. AKM Shafiqul Islam (Advisor)
Abdul Malek, Abdullah
format Learning Object
author Abdul Malek, Abdullah
author_sort Abdul Malek, Abdullah
title Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
title_short Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
title_full Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
title_fullStr Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
title_full_unstemmed Development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
title_sort development of disposable superoxide dismutase biosensor to determined the antioxidant capacity in tea plant
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13352
_version_ 1643790815711985664
score 13.214268

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