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Chemical characterization of different types of herbs using instrumentation methods

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Bibliographic Details
Main Author: Bidattul Syirat, Zainal
Other Authors: Assoc. Prof. Dr Zainab Hamzah (Advisor)
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Herbs
Herbal plant
Orthosiphon stamineus
Misai Kucing
Catharanthus roseus
Vinca rosea
Kemunting Cina
Anticancer drug
Medicinal plant
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13349
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spelling my.unimap-133492011-07-30T05:35:57Z Chemical characterization of different types of herbs using instrumentation methods Bidattul Syirat, Zainal Assoc. Prof. Dr Zainab Hamzah (Advisor) Herbs Herbal plant Orthosiphon stamineus Misai Kucing Catharanthus roseus Vinca rosea Kemunting Cina Anticancer drug Medicinal plant Access is limited to UniMAP community. A simple High Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared (FTIR) methods were developed and validated for the determination of specific polyphenol components such as Rosmarinic Acid (RA), Eupatorin (EUP), Sinensitin (SIN) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (TMF), as well as vincristine and vinblastine, in shoot, leave, stem and root extracts of Orthosiphon stamineus (also known as Misai Kucing) and Catharanthus roseus (also known as Vinca rosea) herb respectively. Separation and quantitative analysis of extracts using Ethanol Extraction, Water Extraction and Pressure Extraction were performed using Shimadzu HPLC System. An isocratic mobile phase consisted of water, methanol and tetrahydrofluoride (50:45:5, v/v) were used in this study. An FTIR analysis was performed to determine the functional groups present in the extracts. The concentrations of the phenolic compounds in the shoot, leave, stem and root extracts were determined using HPLC with ultraviolet detection at 340 nm. The analysis showed that the concentration of RA and EUP in leaves of Misai Kucing was higher than other parts of the plant. Meanwhile, the concentrations of vincristine and vinblastine in each parts of Vinca plant were undetectable. However, Water Extraction technique seems to be the most economical, time-consuming and environmental friendly compared to two other methods. 2011-07-30T05:35:57Z 2011-07-30T05:35:57Z 2011-05 Learning Object http://hdl.handle.net/123456789/13349 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Herbs
Herbal plant
Orthosiphon stamineus
Misai Kucing
Catharanthus roseus
Vinca rosea
Kemunting Cina
Anticancer drug
Medicinal plant
spellingShingle Herbs
Herbal plant
Orthosiphon stamineus
Misai Kucing
Catharanthus roseus
Vinca rosea
Kemunting Cina
Anticancer drug
Medicinal plant
Bidattul Syirat, Zainal
Chemical characterization of different types of herbs using instrumentation methods
description Access is limited to UniMAP community.
author2 Assoc. Prof. Dr Zainab Hamzah (Advisor)
author_facet Assoc. Prof. Dr Zainab Hamzah (Advisor)
Bidattul Syirat, Zainal
format Learning Object
author Bidattul Syirat, Zainal
author_sort Bidattul Syirat, Zainal
title Chemical characterization of different types of herbs using instrumentation methods
title_short Chemical characterization of different types of herbs using instrumentation methods
title_full Chemical characterization of different types of herbs using instrumentation methods
title_fullStr Chemical characterization of different types of herbs using instrumentation methods
title_full_unstemmed Chemical characterization of different types of herbs using instrumentation methods
title_sort chemical characterization of different types of herbs using instrumentation methods
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13349
_version_ 1643790814825938944
score 13.214268

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