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Optimization of glucose oxidase production by using indigenous fungal strain

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Bibliographic Details
Main Author: Nurul Ain, Borhan
Other Authors: Prof. Madya Dr. Dachyar Arbain (Advisor)
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Indigenous fungal strain
Glucose oxidase (GOD)
Gluconic acid
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13143
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spelling my.unimap-131432011-07-11T03:20:21Z Optimization of glucose oxidase production by using indigenous fungal strain Nurul Ain, Borhan Prof. Madya Dr. Dachyar Arbain (Advisor) Indigenous fungal strain Glucose oxidase (GOD) Gluconic acid Access is limited to UniMAP community. Glucose oxidase (β-D-glucose:oxygen 1-oxidoreductase; EC 1.1.2.3.4) catalyzes the oxidation of β-D-glucose to gluconic acid, by utilizing molecular oxygen as an electron acceptor with simultaneous production of hydrogen peroxide. A number of nutritional factors influencing glucose oxidase (EC 1.1.3.4) production by indigenous fungal strain were studied. The synthesis of glucose oxidase by indigenous fungal strain was investigated by using submerged fermentation at 30 ± 2oC and 200 rpm for 110 hours. Primarily, nutritional component were selected by significance of each component with respect to glucose oxidase production was identified by Plackett-Burman design (eight variables including seven nutritional, e.g sodium nitrate (NaNO3), potassium phosphate (KH2PO4), magnesium hydrate sulfate (MgSO4.7H2O), iron hydrate sulfate (FeSO4.7H20), peptone, calcium carbonate (CaCO3), glucose and one dummy or unassigned variable were studied with twelve experiment). The outcome of Plackett-Burman design showed four factors that affected the glucose oxidase production, which are glucose, peptone, NaNO3 and CaCO3. 2011-07-11T03:20:21Z 2011-07-11T03:20:21Z 2010-05 Learning Object http://hdl.handle.net/123456789/13143 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Indigenous fungal strain
Glucose oxidase (GOD)
Gluconic acid
spellingShingle Indigenous fungal strain
Glucose oxidase (GOD)
Gluconic acid
Nurul Ain, Borhan
Optimization of glucose oxidase production by using indigenous fungal strain
description Access is limited to UniMAP community.
author2 Prof. Madya Dr. Dachyar Arbain (Advisor)
author_facet Prof. Madya Dr. Dachyar Arbain (Advisor)
Nurul Ain, Borhan
format Learning Object
author Nurul Ain, Borhan
author_sort Nurul Ain, Borhan
title Optimization of glucose oxidase production by using indigenous fungal strain
title_short Optimization of glucose oxidase production by using indigenous fungal strain
title_full Optimization of glucose oxidase production by using indigenous fungal strain
title_fullStr Optimization of glucose oxidase production by using indigenous fungal strain
title_full_unstemmed Optimization of glucose oxidase production by using indigenous fungal strain
title_sort optimization of glucose oxidase production by using indigenous fungal strain
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13143
_version_ 1643790758654771200
score 13.222552

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