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Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method

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Main Author: Fan, Chao Way
Other Authors: Siti Jamilah Hanim Mohd Yusof
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis 2011
Subjects:
Antioxidant
Tocopherols
Pangium edule
Extraction
High performance liquid chromatography (HPLC)
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/13101
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spelling my.unimap-131012011-07-08T01:43:21Z Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method Fan, Chao Way Siti Jamilah Hanim Mohd Yusof Antioxidant Tocopherols Pangium edule Extraction High performance liquid chromatography (HPLC) Access is limited to UniMAP community. The market demand of the tocopherols in the antioxidant product was relatively high as tocopherols were proved to be the most powerful antioxidant compared others. However, the global supply of tocopherols from the natural sources (edible oil distillate) has not meet the demand of public. Tocopherols is the most powerful antioxidant among the vitamin E group. In this study, determination of tocopherols content in the seeds of Pangium edule using high performance liquid chromatography (HPLC) is reported. The extraction of antioxidant was done by using the solid liquid extraction method of saponification with 70% of sodium hydroxide (NaOH). Process extraction was done by saponification of samples with methanolic and ethanolic solution of ascorbic acid and extraction of the tocopherol with the mixture of acetronitrile and methanol at 50:50 v/v ratio. Furthermore, the presence of the tocopherols in the seeds was verified by HPLC-UV and the DPPH detection was used to determine the antioxidant activity. The tocopherols levels determined in the seeds by HPLC with UV in the ethanolic solution of ascorbic acid (2.3118 µg/ml) are reported to be higher than those in the methanolic solution (1.6699 µg/ml). However, the antioxidant activity detected in the methanolic acid is higher indicating that there are many others antioxidants contained in the seeds besides tocopherols. As a conclusion, from this study, it is obvious that solid liquid extraction method offers an acceptable performance for the determination of antioxidant from the seeds of Pangium edule. The potential of Pangium edule for antioxidant resource was successfully demonstrated by HPLC and DPPH analysis. 2011-07-08T01:43:21Z 2011-07-08T01:43:21Z 2010-05 Learning Object http://hdl.handle.net/123456789/13101 en Universiti Malaysia Perlis School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antioxidant
Tocopherols
Pangium edule
Extraction
High performance liquid chromatography (HPLC)
spellingShingle Antioxidant
Tocopherols
Pangium edule
Extraction
High performance liquid chromatography (HPLC)
Fan, Chao Way
Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
description Access is limited to UniMAP community.
author2 Siti Jamilah Hanim Mohd Yusof
author_facet Siti Jamilah Hanim Mohd Yusof
Fan, Chao Way
format Learning Object
author Fan, Chao Way
author_sort Fan, Chao Way
title Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
title_short Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
title_full Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
title_fullStr Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
title_full_unstemmed Determination of antioxidant compounds from the seeds of Pangium edule by solid liquid extraction method
title_sort determination of antioxidant compounds from the seeds of pangium edule by solid liquid extraction method
publisher Universiti Malaysia Perlis
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/13101
_version_ 1643790747219001344
score 13.160551

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