Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms
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2014
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my.unikl.ir-71852014-07-14T01:44:49Z Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms Iram Liaqat Robert Thomas Bachmann Robert G. J. Edyvean UniKL MICET Full text articles available in SpringerLink. (ISI Journal) The quorum sensing (QS) dependent behaviour of micro-organisms, in particular expression of virulence genes, biofilm formation and dispersal, have provided impetus for investigating practical approaches to interfere with microbial QS. This study tests Halomonas pacifica and Marinobacter hydrocarbonoclasticus, two halophilic marine micro-organism, for their AI-2 dependent QS signalling and the effect of two well-known quorum-sensing inhibitors (QSIs), patulin and penicillic acid, on biofilm formation. We report, for the first time, the successful amplification of a putative luxS gene in H. pacifica using degenerated primers and AI-2 dependent QS as well as inhibition using QSIs. Penicillic acid had a strong inhibitory effect on AI-2 induction of H. pacifica at non-growth inhibitory concentrations, while patulin has an adverse effect only at the highest concentration (25 μM). QSIs effect on biofilm forming capability was isolate specific, with maximum inhibition at 25 μM of patulin in H. pacifica. In M. hydrocarbonoclasticus, no adverse effects were noted at any tested concentration of either QSIs. Detection of bioluminescence and the presence of a putative luxS gene provide biochemical and genetic evidence for the production of a signalling molecule(s) which is the essential first step in characterizing H. pacifica QS. This study highlights the importance of AI-2 dependent QS in a marine setting, not previously reported. It further suggests that QSI compounds must be selected in the specific system in which they are to function, and they cannot easily be transferred from one QS system to another. 2014-07-14T01:44:49Z 2014-07-14T01:44:49Z 2013 Liaqat, I., Bachmann, R., & Edyvean, R. J. (2014). Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms. Current Microbiology, 68(3), 342–351. doi:10.1007/s00284-013-0484-5 1432-0991 http://link.springer.com/article/10.1007/s00284-013-0484-5 http://localhost/xmlui/handle/123456789/7185 Current Microbiology; Springer US |
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Full text articles available in SpringerLink. (ISI Journal) |
| format |
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| author |
Iram Liaqat Robert Thomas Bachmann Robert G. J. Edyvean UniKL MICET |
| spellingShingle |
Iram Liaqat Robert Thomas Bachmann Robert G. J. Edyvean UniKL MICET Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| author_facet |
Iram Liaqat Robert Thomas Bachmann Robert G. J. Edyvean UniKL MICET |
| author_sort |
Iram Liaqat |
| title |
Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| title_short |
Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| title_full |
Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| title_fullStr |
Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| title_full_unstemmed |
Type 2 Quorum Sensing Monitoring, Inhibition and Biofilm Formation in Marine Microrganisms |
| title_sort |
type 2 quorum sensing monitoring, inhibition and biofilm formation in marine microrganisms |
| publisher |
Springer US |
| publishDate |
2014 |
| url |
http://link.springer.com/article/10.1007/s00284-013-0484-5 http://localhost/xmlui/handle/123456789/7185 |
| _version_ |
1644484878154072064 |
| score |
13.211869 |