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Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes

In order to harness the conductivity of Lambda DNA (λ-DNA), the conduction of DNA molecules needs to be determined by DEP trapping experiments. The electrical properties of DNA molecules have been characterized by crossover frequency method. In this work a YOYO®-1 Iodide (purchased from Invitrogen L...

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Main Authors: Mohamad, Ahmad Sabry (UniKL BMI), Jeynes, Jonathan Charlie, Hughes, Michael Pycraft
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Published: 2014
Subjects:
Dielectrophoresis
Lambda DNA
quadruple electrodes
Online Access:http://localhost/xmlui/handle/123456789/7057
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id my.unikl.ir-7057
record_format eprints
spelling my.unikl.ir-70572014-06-12T01:32:28Z Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes Mohamad, Ahmad Sabry (UniKL BMI) Jeynes, Jonathan Charlie Hughes, Michael Pycraft Dielectrophoresis Lambda DNA quadruple electrodes In order to harness the conductivity of Lambda DNA (λ-DNA), the conduction of DNA molecules needs to be determined by DEP trapping experiments. The electrical properties of DNA molecules have been characterized by crossover frequency method. In this work a YOYO®-1 Iodide (purchased from Invitrogen Ltd UK) shows a thousand-fold increase in its green fluorescence when bound to λ-DNA. The double stranded λ-DNA with 48.5 kbp trapped at the high-field region of gold quadruple-electrodes and acting as negative DEP. To ensure the correct system functionalities the simulation of electric fields were performed. The results demonstrate positively behaved particles would be pulled to electrode tips, while negatively behaved particles push away to the regions between the electrodes. Malaysian Government under Grant Skim Tindikan MARA UniKL BMI 2014-06-03T08:56:55Z 2014-06-03T08:56:55Z 2014-06-03 http://localhost/xmlui/handle/123456789/7057
institution Universiti Kuala Lumpur
building UniKL Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kuala Lumpur
content_source UniKL Institutional Repository
url_provider http://ir.unikl.edu.my/
topic Dielectrophoresis
Lambda DNA
quadruple electrodes
spellingShingle Dielectrophoresis
Lambda DNA
quadruple electrodes
Mohamad, Ahmad Sabry (UniKL BMI)
Jeynes, Jonathan Charlie
Hughes, Michael Pycraft
Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
description In order to harness the conductivity of Lambda DNA (λ-DNA), the conduction of DNA molecules needs to be determined by DEP trapping experiments. The electrical properties of DNA molecules have been characterized by crossover frequency method. In this work a YOYO®-1 Iodide (purchased from Invitrogen Ltd UK) shows a thousand-fold increase in its green fluorescence when bound to λ-DNA. The double stranded λ-DNA with 48.5 kbp trapped at the high-field region of gold quadruple-electrodes and acting as negative DEP. To ensure the correct system functionalities the simulation of electric fields were performed. The results demonstrate positively behaved particles would be pulled to electrode tips, while negatively behaved particles push away to the regions between the electrodes.
format
author Mohamad, Ahmad Sabry (UniKL BMI)
Jeynes, Jonathan Charlie
Hughes, Michael Pycraft
author_facet Mohamad, Ahmad Sabry (UniKL BMI)
Jeynes, Jonathan Charlie
Hughes, Michael Pycraft
author_sort Mohamad, Ahmad Sabry (UniKL BMI)
title Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
title_short Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
title_full Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
title_fullStr Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
title_full_unstemmed Dielectrophoretic Collection of Lambda-Dna Using Quadruple Electrodes
title_sort dielectrophoretic collection of lambda-dna using quadruple electrodes
publishDate 2014
url http://localhost/xmlui/handle/123456789/7057
_version_ 1644484849035116544
score 13.214268

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